A New Pulsed FAB Control System Coupled with an Array Detector on a Four-Sector Tandem Mass Spectrometer

新型脉冲 FAB 控制系统与四扇区串联质谱仪上的阵列检测器相结合

• 批准号: 04558022
• 负责人: SHIMONISHI Yasutsugu
• 金额: $ 6.4万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04558022/
• 关键词: Pulsed FAB; Array detector; Four-sector tandem mass spectrometer; Function generator; Initial ionization event; Sample lifetime; MS; MS sequencing; Femtomole; パルスイオン化; 高感度質量分析; MS; MSスペクトル; 蛋白質構造解析; 質量分析; アレイ検出器; 高感度測定

A pulsed FAB has been proposed to be a powerful technique for dramatic enhancement in sensitivity and increase in sample lifetimes. Here we developed a new pulsed FAB control system coupled with a photodiode-based array detector on a four-sector tenden mass spectrometer.The trigger pulse initially generated from the array detector control system is transmitted to the data acquisition processor via an optical fiber line, and them, to a function generator. The function generator is designed to provide a set of pulses of various repetitive frequencies and duty cycles. A high voltage power supply combined with the function generator allows a primary ion beam to be accelerated at up to a 6-kV potential with a quick rising and falling time less than 1 millisecond. The pulsing of primary ion beam is synchronized with start-up of the data acquisition on the array detector.When the system was tested on the second MS using a pulsed Xe atom beam, the protonated molecular ion peaks of peptides were obtained with 10 times higher intensity than those by a continuous FAB in the initial ionization event. The detection limits for angiotensin I (m/z 1296.7) and substance P (1347.7) were about 10 femtomoles. This new pulsed FAB control system was further demonstrated to be effective for reducing sample amounts required for MS/MS sequencing of peptides to the subfemtomole order.

脉冲FAB已被认为是显著提高灵敏度和延长样品寿命的一种强有力的技术。在四区展宽质谱仪上研制了一种新型的脉冲FAB控制系统，该系统与基于光电二极管的阵列探测器相耦合，最初由阵列探测器控制系统产生的触发脉冲通过光纤线传输到数据采集处理器，然后再传输到函数发生器。该函数发生器被设计成提供各种重复频率和占空比的脉冲集合。与函数发生器相结合的高压电源允许一次离子束以高达6千伏的电位加速，快速上升和下降时间不到1毫秒。一次离子束的脉冲与阵列探测器上数据采集的启动同步，当系统在二次质谱仪上使用脉冲Xe原子束进行测试时，在初始电离事件中获得了比连续FAB高10倍的质子化多肽分子离子峰。血管紧张素I(m/z 1296.7)和P物质(1347.7)的检出限约为10毫微克分子。这种新的脉冲FAB控制系统被进一步证明是有效的，可以将多肽的MS/MS测序所需的样本量减少到亚毫微米级。

项目成果

Kanaji,T.: "Primary Structure of Microbial Transglutaminase from Streptoverticillium sp.Strain S-8112." J.Biol.Chem.268. 11565-11572 (1993)

Kanaji,T.：“来自链轮丝菌菌株 S-8112 的微生物转谷氨酰胺酶的一级结构。”

Takao, T.and Shimonishi, Y.: "Determination of Posttranslational Modifications by Mass Spectrometry." "Methods in Protein Sequence Analysis" Eds.Imahori, K.and Sakiyama, F.Plenum. 157 (1993)

Takao, T. 和 Shimonishi, Y.：“通过质谱法测定翻译后修饰”。

Fukuda,H.: "Sequence Verification of Peptide Nucleic Acids Using Positive-Ion Fast Atom Bomberdment Tandem Mass Spectrometry" Abstracts of the 42nd ASMS Conference on Mass Spectrometry and Allied Topics,Chicago. (in press). (1994)

Fukuda,H.：“使用正离子快原子轰击串联质谱法对肽核酸进行序列验证”第 42 届 ASMS 质谱及相关主题会议摘要，芝加哥。

Kokame,K.: "Lipid Modification at the N Terminus of Photoreceptor G-Protein alpha-Subunit." Nature. 359. 749-51 (1992)

Kokame,K.：“光感受器 G 蛋白 α 亚基 N 末端的脂质修饰。”

Kokame,Y.Fukada,T.Yoshizawa & Y.Shimonishi: "Lipid modification at the N terminus of photo-receptor G-protein α-subunit" Nature. 359. 749-752 (1992)

Kokame、Y. Fukada、T. Yoshizawa 和 Y. Shimonishi：“光受体 G 蛋白 α 亚基 N 末端的脂质修饰”《自然》359. 749-752 (1992)。