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A Study on Concentration method for Microorganisms in Water with Electropositive Filter

正电过滤器浓缩水中微生物方法的研究

基本信息

批准号：
02650386
负责人：
HIRATA Tsuyoshi
金额：
$ 1.41万
依托单位：
Azabu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

An experimental study for concentrating the microorganisms from water using electropositive filter (Zeta plus Virosorb 1MDS, Cuno Inc.) was conduced. Microorganisms tested were Escherichia coli, Clostridium perfringens, and coliphages of Q-beta, MS2 and an environmental strain. Results obtained are summarized as follows ; (1) Recovery rates of the electropositive filter for Clostridium perfringens and Escherichia coli were greater than 95% and 80% respectively. (2) Recovery of coliphages was low with 20-50% for Q-beta and 30-50% for MS2 in wide range of pH 4-9. (3) Recovery rates for unidentified strain isolated from river water were decreased with increased pH and the values of the rates were greater than 90% at pH 4-6, around 70% at pH 7 and 40-50% at pH 8-9. (4) From these results, it was suggested that the electropositive filter was applicable for concentrating bacteria from water without any pretreatment, while pH adjusting to less than 6 would be necessary for phage.Additionally, a trial of isoelectric focussing to determine the isoelectric point of phages was conducted using a commercially available equipment divided and separated into twenty small chambers by membrane filters (Rotophore, Bio-Rad Inc.). From this trial, it was concluded that the system used would be inadequate for the determination of isoelectric point of phages in water because these phages were recovered with extremely low rate after isoelectric focussing.

采用Zeta + Virosorb 1MDS公司生产的正电过滤器对水中微生物进行了富集实验研究。检测的微生物为大肠杆菌、产气荚膜梭菌、q - β、MS2和一种环境菌株的噬菌体。所得结果总结如下：(1)电正过滤器对产气荚膜梭菌和大肠杆菌的回收率分别大于95%和80%。(2)在pH 4 ~ 9范围内，q - β和MS2的噬菌体回收率较低，分别为20 ~ 50%和30 ~ 50%。(3)从河水中分离的未识别菌株的回收率随pH值的升高而降低，pH值4 ~ 6时回收率大于90%，pH值7时约为70%，pH值8 ~ 9时为40 ~ 50%。(4)从以上结果可以看出，电正过滤器适用于不经预处理的水中细菌的富集，而噬菌体的富集则需要将pH调节到小于6。此外，使用市售设备进行等电聚焦试验，以确定噬菌体的等电点，这些设备由膜过滤器（Rotophore, Bio-Rad Inc.）分成20个小室。从这个试验中得出结论，所使用的系统不适合测定水中噬菌体的等电点，因为这些噬菌体在等电聚焦后的回收率极低。