Development and application of automatic analyzer for sensitive catalytic analysis

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A flow system with air carrier was developed as a new automatic analyzer for sensitive catalytic analysis. The analyzer was applied to determine iodide ion by measurement of a coloration rate constant of a new redox reaction using iodide-catalyzed oxidation of Chlorpromazine (CP) with H_2O_2 in H_2SO_4 (the pseudo zero-order reaction).(1) The analyzer consists of injectors and a laboratory-made micro cell, which are connected in series by PTFE tubing with 1 and 2 mm i. d. Each 150 mul of sample and two reagent solutions were introduced into the flow system by pumping and siphoning. The precision of siphon injection was 0.2%, comparable to a manual syringe injection. The three air-segmented solution zones were carried by air pumping, into the cell, in which the solutions were mixed to initiate a reaction and measure the reaction rate constant. Reproducible mixing was performed within an error of 1% in absorbance measurement of rfethyl Orange solution mixed with water and CP solution.(2) By a manual batch technique, the reaction condition of reactant concentrations and reaction temperature were optimized for sensitive determination of iodide ion.(3) The detection limit of iodide ion using the analyzer was 3 ng, which was lower than that of about 50 ng by the batch technique.