Preparation of adsorbents having a high affinity for endotoxins with polyamino acid

聚氨基酸制备内毒素高亲和力吸附剂

• 批准号: 02650669
• 负责人: HIRAYAMA Chuich
• 金额: $ 1.15万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02650669/
• 关键词: endotoxin; Bordetella pertussis; poly (gamma-methyl L-glutamate); amino group; antigen; filamentous hemagglutinin; pertussis toxin; polyamino acid; ポリ(αーメチル Lーグルタメ-ト); 疎水性相互作用; アフィニティ-; 破傷風トキソィド; ポリ(γーメチル Lーグルタメ-ト); 排除限界分子量

Preparations of adsorbents having a high affinity for endotoxins in the culture supernatant fluids of Bordetella pertussis were investigated. When poly (gamma-methyl L-glutamate) (PMLG) was used as a matrix and amino groups was used as the ligand, the highest affinity for endotoxin was attained even at high ionic strength (mu=0.2-0.4). PMLG beads. containing amino groups of about 3.2 meq/g selectively removed endotoxin in culture supernatant of B. pertussis without affecting the protective antigens. It was demonstrated that 1 ml of the wet adsorbent adsorbed 4.5 mg of endotoxin. The beads of PMLG derivatives, therefore, are considered to be useful adsorbent for the removal of endotoxin from pertussis vaccine affecting neither filamentous hemagglutinin nor pertussis toxin.

研究了对百日咳博德特氏菌培养上清液中的内毒素具有高亲和力的吸附剂的制备。当使用聚（γ-甲基L-谷氨酸）（PMLG）作为基质并使用氨基作为配体时，即使在高离子强度（μ=0.2-0.4）下也能获得对内毒素的最高亲和力。 PMLG 珠。含有约3.2meq/g的氨基，选择性去除百日咳博德特氏菌培养上清液中的内毒素，而不影响保护性抗原。结果表明，1ml湿吸附剂可吸附4.5mg内毒素。因此，PMLG衍生物珠被认为是用于去除百日咳疫苗中的内毒素的有用吸附剂，既不影响丝状血凝素也不影响百日咳毒素。

项目成果

平山 忠一: "Regulation of poreーsize distribution of poly(γーmethyl Lーglutamate)spheres as a gel permeation chromatography packings" Polymer Journal. 22. 614-619 (1990)

Chuichi Hirayama：“作为凝胶渗透色谱填料的聚（γ-甲基 L-谷氨酸）球体的孔径分布调节”聚合物杂志 22. 614-619 (1990)。

Chuichi Hirayama, Masayo Sakata, Yukihiro Ohkura, Hirotaka Ihara, and Kunio Ohkuma: "Removal of endotoxin from culture supernatant of Bordetella pertussis with aminated poly (gamma-methyl L-glutmate) spherical beads" Chemical & Pharmaceutical Bulletin.

Chuichi Hirayyama、Masayo Sakata、Yukihiro Ohkura、Hirotaka Ihara 和 Kunio Ohkuma：“用胺化聚（γ-甲基 L-谷氨酸）球形珠去除百日咳博德特氏菌培养物上清液中的内毒素”化学

平山 忠一: "Development of macroporous packings for aqueous high flow rate liquid chromatography.Approach on the viewpoint of materials chemistry" The Japan Society for Analitical Chemistry. 7. 527-536 (1991)

Chuichi Hirayama：“用于水性高流速液相色谱的大孔填料的开发。材料化学观点的方法”日本分析化学学会 7. 527-536 (1991)。

平山 忠一: "Regulation of poreーsize distribution of poly(γーmethl Lーglutamate)sphers as a gel permeation chromatography packings" Polymer Journal. 22. 614-619 (1990)

Chuichi Hirayama：“作为凝胶渗透色谱填料的聚（γ-甲基 L-谷氨酸）球的孔径分布调节”聚合物杂志 22. 614-619 (1990)。

平山 忠一: "Remoal of endotoxins using macroprous spherical poly(γーmethl Lーglutamate)beads and their derivatives" Journal of Chromatography. 530. 148-153 (1990)

Tadakazu Hirayama：“使用大球状聚（γ-甲基 L-谷氨酸）珠及其衍生物去除内毒素”《色谱杂志》530. 148-153 (1990)。