Production of Acetic Acid from Carbon Dioxide by Immobilized Acetobacterium woodi in Bubble-Column Bioceactor

厌氧细菌伍氏醋酸杆菌（Acetobacterium woodii）能从CO_2中产生乙酸。该菌的利用不仅对C_1化学的发展，而且对大气中CO_2的固定都具有巨大的价值。本研究的目的是找出A.为了提高A. woodii对CO_2的利用率，我们研制了一台300 ml的多孔板气分布鼓泡塔型生物反应器。woodii（ATCC 29683）。A.的基本生长特性和最佳操作条件。woodii（温度、H_2/CO_2比、培养基等）通过使用具有往复振荡的试管来测定。研究了A.使用两种类型的厌氧微生物，悬浮的和固定化的细胞，在鼓泡塔生物反应器中检测伍氏菌。在固定化细胞系统中，细菌被截留在精氨酸钙细胞中。更高的醋酸盐生产率， ...更多信息 从实用的观点来看，需要建立这种细菌的长距离连续培养方法。人们必须找到一种合适的方法将细胞从培养基中分离出来。细菌的絮凝将有助于使用悬浮细胞代替细胞的固定化方法的连续高密度培养。A.絮凝通过对不同絮凝剂的絮凝效果进行研究，实现了对A. woodii的高密度培养。woodii用于醋酸盐生产。具有强正电荷的阳离子合成高分子絮凝剂对A. woodii。将该方法应用于鼓泡塔生物反应器中醋酸的连续生产，证实了其有效性。这种培养方法不仅对于简化过程，而且对于利用分离细胞和液体内容物所需的能量是有价值的。少

An anaerobic bacterium, Acetobacterium woodii, can produce acetic acid from CO_2. The utilization of such bacteria is of enormous value, not only for the development of C1 chemistry but also for the fixation of CO_2 in the atmosphere. The object of this study is to find the problems to be solved in the industrial application of A. woodii by using the bioreactors.We have developed a 300 ml bubble-column type bioreactor with the porous plate as gas distributor for the advantageous utilization of CO_2 by A. woodii (ATCC 29683). Basic growth characteristics and some optimal operational conditions for A. woodii (temperature, H_2/CO_2 ratio, medium et al.) were determined by using test tubes with reciprocal shaking. The production of acetic acid from H_2 and CO_2 by A. woodii was examined in the bubble-column bioreactor using two types of anaerobes, suspended and immobilized cells. In the immobilized-cell system, bacteria were entrapped in calcium arginate cell. Higher acetate productivity w … More as found in the culture of suspended cells.From the practical viewpoint, the establishment of a long-range continuous cultivation method of such bacteria will be required. One has to find a proper method of the separation of the cells from their media. Flocculation of bacteria will assist in the continuous high density cultivation using suspended cells in place of the immobilization methods for the cells. Flocculation of A. woodii by a variety of flocculants was tested to achieve high density cultivation using suspended cells of A. woodii for acetate production. Cationic synthetic polymeric flocculants having a strong positive charge were found to be highly effective to flocculate A. woodii. This approach was applied to the continuous production of acetic acid in the bubble-column bioreactor, and its usefulness was confirmed. This cultivation method is valuable not only for the simplification of the process, but also for the utilization of the energy required to the separation of the cells and the liquid contents. Less