Production of Acetic Acid from Carbon Dioxide by Immobilized Acetobacterium woodi in Bubble-Column Bioceactor

固定化伍迪醋杆菌在鼓泡塔生物反应器中二氧化碳生产乙酸

• 批准号: 02650704
• 负责人: KOIDE Kozo
• 金额: $ 1.28万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02650704/
• 关键词: Acetobactreium woodii; Carbon dioxide; Autotrophic bacteria; Acetic acid; Bioreactor; Anaerobic bacteria; Immobilized cell; Flocculation; Acetobacterium woodii; Carbon dioxide; Autotrophic bacteria; Acetic acid; Bioreactor; Anaerobic bacteria

An anaerobic bacterium, Acetobacterium woodii, can produce acetic acid from CO_2. The utilization of such bacteria is of enormous value, not only for the development of C1 chemistry but also for the fixation of CO_2 in the atmosphere. The object of this study is to find the problems to be solved in the industrial application of A. woodii by using the bioreactors.We have developed a 300 ml bubble-column type bioreactor with the porous plate as gas distributor for the advantageous utilization of CO_2 by A. woodii (ATCC 29683). Basic growth characteristics and some optimal operational conditions for A. woodii (temperature, H_2/CO_2 ratio, medium et al.) were determined by using test tubes with reciprocal shaking. The production of acetic acid from H_2 and CO_2 by A. woodii was examined in the bubble-column bioreactor using two types of anaerobes, suspended and immobilized cells. In the immobilized-cell system, bacteria were entrapped in calcium arginate cell. Higher acetate productivity w … More as found in the culture of suspended cells.From the practical viewpoint, the establishment of a long-range continuous cultivation method of such bacteria will be required. One has to find a proper method of the separation of the cells from their media. Flocculation of bacteria will assist in the continuous high density cultivation using suspended cells in place of the immobilization methods for the cells. Flocculation of A. woodii by a variety of flocculants was tested to achieve high density cultivation using suspended cells of A. woodii for acetate production. Cationic synthetic polymeric flocculants having a strong positive charge were found to be highly effective to flocculate A. woodii. This approach was applied to the continuous production of acetic acid in the bubble-column bioreactor, and its usefulness was confirmed. This cultivation method is valuable not only for the simplification of the process, but also for the utilization of the energy required to the separation of the cells and the liquid contents. Less

一种厌氧细菌，伍氏醋酸杆菌，可以从CO_2中产生乙酸。这类细菌的利用不仅对C1化学的发展，而且对大气中CO_2的固定具有巨大的价值。本研究的目的是寻找利用该生物反应器在木犀草工业应用中需要解决的问题。为使伍氏弓形虫（ATCC 29683）对CO_2有较好的利用，研制了一种300 ml多孔板配气的气泡柱式生物反应器。采用倒摇试管法，确定了伍迪的基本生长特性和最佳操作条件（温度、H_2/CO_2比、培养基等）。在气泡柱生物反应器中，采用悬浮和固定化两种厌氧菌，研究了伍伍草由H_2和CO_2生成乙酸的过程。在固定化细胞体系中，细菌被包裹在精氨酸钙细胞中。在悬浮细胞培养中发现了更高的醋酸盐产量。从实际应用的角度来看，需要建立一种这种细菌的长期连续培养方法。必须找到一种适当的方法把细胞从培养基中分离出来。细菌絮凝将有助于使用悬浮细胞代替固定化方法进行连续高密度培养。试验了多种絮凝剂对伍伍木的絮凝作用，以实现伍伍木悬浮细胞的高密度培养。阳离子型合成高分子絮凝剂具有较强的正电荷，对木桐具有较好的絮凝效果。将该方法应用于气泡塔生物反应器中醋酸的连续生产，验证了该方法的有效性。这种培养方法不仅简化了过程，而且还利用了分离细胞和液体内容物所需的能量。少

项目成果

Takahiro Suzuki et al.: ""Kinetics of Production of Acetic Acid from Carbon Dioxide by Acetobacterium woodii in Bubble-Column Bioreactor"" J. Chemical Engineering of Japan. 25. 106-108 (1992)

Takahiro Suzuki 等人：“在泡罩塔生物反应器中伍氏醋杆菌从二氧化碳中生产乙酸的动力学”，J. Chemical Engineering of Japan。

Takahiro Suzuki: "Flocculation of Acetobacterium woodii for Continuous Conversion of Carbon Dioxide to Acetic Acid"

Takahiro Suzuki：“伍氏醋酸杆菌絮凝，用于二氧化碳连续转化为乙酸”