DEVELOPMENT OF MULTIPHASE BIOREACTORS INVOLVING ORGANIC SOLVENT WITH SILICONE-IMMOBILIZED BIOCATALYSTS

涉及有机溶剂和有机硅固定生物催化剂的多相生物反应器的开发

• 批准号: 02650708
• 负责人: KAWAKAMI Koei
• 金额: $ 1.34万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02650708/
• 关键词: Immobilized Biocatalyst; Mixed Matrix; Nonaqueous Media; Bioreactor; Silicone Polymer; Epoxidation; Bioconversion; Alcohol Dehydrogenase; バイオリアクタ-; シリコ-ンポリマ-; バイオコンバ-ジョン; アルコ-ルデヒドロゲナ-ゼ

The development of supports for immobilized enzymes and microbial cells in non-aqueous systems is an area of high interest. We applied hydrophobic silicone polymer to immobilization by entrapment of biocatalysts operating in organic media.1.Horse liver alcohol dehydrogenase,which was once solubilized in a small amount of water,or impregnated in water-containing porous particles,was immobilized in a silicone polymer sheet. This configuration also enabled to perform a simple packed-bed operation for the enzymatic oxidation of alcohol and reduction of aldehyde with a coupled-substrate coenzyme recycling in organic media.2.Immobilization of Nocardia corallina was examined for epoxidation of liquid alkenes such as 1-tetradecene,1-octene and styrene to produce corresponding epoxides in the presence of n-hexadecane as the organic solvent. The silicone polymer in which droplets of the cell suspension were finely dispersed,was the most suitable material retaining high epoxide productivity. In o … More rder to change hydrophobicity-hydrophilicity balance of the support over a wide range,it was effective to immobilize the cells in a mixed matrix composed of silicone polymer and calcium alginate gel. In the support containing excess silicone over 60%,fine particles of the alginate were dispersed in the continuous silicone phase,and in the case of alginate over 40% the phase configuration was inversed. The optimum composition of the mixed matrix was 80-90% silicone+20-10% alginate for the production of 1,2-epoxytetradecane,40-50% silicone+ 60-50% alginate for 1,2-epoxyoctane,and almost 0% silicone+100% alginate for styrene oxide. This significant change of the optimum composition was closely related to the degree of substrate inhibition. Such an optimized composite-immobilized cells offered the epoxide productivity superior to the free cells in the two-liquid phase after a longer period of batch operation,and enabled to perform a relatively simple operation of a continuous three-phase bioreactor system. Less

在非水系统中固定化酶和微生物细胞的支架的发展是一个高度感兴趣的领域。我们将疏水性有机硅聚合物应用于有机介质中生物催化剂的包埋固定化。将马肝醇脱氢酶溶解于少量水中，或浸渍于含水的多孔颗粒中，固定在硅胶聚合物片上。该配置还可以在有机介质中通过偶联底物辅酶循环进行醇酶氧化和醛还原的简单填充床操作。以正十六烷为有机溶剂，研究了珊瑚诺卡菌对液体烯烃（1-十四烯、1-辛烯和苯乙烯）的环氧化反应。细胞悬浮液液滴分散良好的有机硅聚合物是最合适的材料，具有较高的环氧化物生产率。为了在更大范围内改变载体的亲疏水性平衡，将细胞固定在由有机硅聚合物和海藻酸钙凝胶组成的混合基质中是有效的。在硅盐含量超过60%的载体中，海藻酸盐的细颗粒分散在连续硅相中，而在硅盐含量超过40%的情况下，相配置相反。合成1,2-环氧十四烷的最佳混合基质为80-90%有机硅+20-10%海藻酸盐，合成1,2-环氧辛烷的最佳混合基质为40-50%有机硅+ 60-50%海藻酸盐，合成氧化苯乙烯的最佳混合基质为几乎0%有机硅+100%海藻酸盐。最佳组成的这种显著变化与底物抑制程度密切相关。优化后的复合固定化细胞经过较长时间的间歇操作后，环氧化物的产率优于两液相的游离细胞，并且可以在连续三相生物反应器系统中进行相对简单的操作。少

项目成果

K.Kawakami: "Characterization of production of 1,2-epoxyoctane from 1-octene by nongrowing cells of Nocardia corallina B-276 in aqueous-organic media" Ann.N.Y.Acad.Sci. Vol.613. 707-711 (1990)

K.Kawakami：“珊瑚诺卡氏菌 B-276 在水性有机介质中非生长细胞从 1-辛烯生产 1,2-环氧辛烷的表征”Ann.N.Y.Acad.Sci。

K.Kawakami: "Reaction characteristics of microbial cells immobilized in a mixed matrix of silicone polymer and aqueous medium" “Physiology of immobilized cells",de Bont et al.,eds.,Elsevier Sci.Publ.,B.V.439-444 (1990)

K. Kawakami：“固定在有机硅聚合物和水性介质的混合基质中的微生物细胞的反应特征”，“固定细胞的生理学”，de Bont 等人编辑，Elsevier Sci Publ.，B.V.439-444（1990 年） ）

K.Kawakami: "Reaction characteristics of microbial cells immobilized in a mixed matrix of silicone polymer and aqueous medium" “Physiology of immobilized cells",de Bont et al.,eds.,Elsevier Sci.Publ.439-444 (1990)

K. Kawakami：“固定在有机硅聚合物和水性介质的混合基质中的微生物细胞的反应特征”，“固定细胞的生理学”，de Bont 等人，编辑，Elsevier Sci，439-444（1990）。

K.Kawakami: "Application of silicone polymer to immobilization of biocatalysts and development of multiphase bioreactors" Proc.APBioChEC'90. 513-516 (1990)

K.Kawakami：“有机硅聚合物在生物催化剂固定化和多相生物反应器开发中的应用”Proc.APBioChEC90。

K.Kawakami: "Immobilization of microbial cells in a mixed matrix of silicone polymer and calcium alginate gel:epoxidation of lーoctene by Nocardia corallina Bー276 in organic media" Biotechnol.Prog.6. 357-362 (1990)

K. Kawakami：“微生物细胞在有机硅聚合物和海藻酸钙凝胶的混合基质中的固定化：珊瑚诺卡氏菌 B-276 在有机介质中环氧化卢辛烯”Biotechnol.Prog.6 (1990)。