DEVELOPMENT OF MULTIPHASE BIOREACTORS INVOLVING ORGANIC SOLVENT WITH SILICONE-IMMOBILIZED BIOCATALYSTS

非水体系中固定化酶和微生物细胞的载体的开发是一个高度关注的领域。将疏水性有机硅聚合物用于有机介质中生物催化剂的包埋固定化。1.将马肝醇脱氢酶（HAD）固定在有机硅聚合物片上。该结构还可实现简单的填充床操作，用于乙醇的酶促氧化和醛的酶促还原。2.以正十六烷为有机溶剂，考察了固定化Corcardia Corcardia对1-十四烯、1-辛烯和苯乙烯等液态烯烃的环氧化反应。细胞悬浮液的液滴精细分散在其中的硅酮聚合物是保持高环氧化物生产率的最合适的材料。在o ...更多信息 为了在较宽范围内改变载体的疏水-亲水平衡，将细胞包埋在由硅酮聚合物和海藻酸钙凝胶组成的混合基质中是有效的。当载体中有机硅含量超过60%时，海藻酸钠微粒分散在连续的有机硅相中，当载体中海藻酸钠含量超过40%时，相结构发生逆转。对于1，2-环氧十四烷的生产，最佳混合基质的组成为80 - 90%的有机硅+20-10%的海藻酸钠;对于1，2-环氧辛烷的生产，最佳混合基质的组成为40 - 50%的有机硅+60-50%的海藻酸钠;这种最佳组成的显著变化与底物抑制程度密切相关。这种优化的复合固定化细胞在较长时间的分批操作后提供了比双液相中的游离细胞更优越的环氧化物生产率上级，并且能够进行连续三相生物反应器系统的相对简单的操作。少

The development of supports for immobilized enzymes and microbial cells in non-aqueous systems is an area of high interest. We applied hydrophobic silicone polymer to immobilization by entrapment of biocatalysts operating in organic media.1.Horse liver alcohol dehydrogenase,which was once solubilized in a small amount of water,or impregnated in water-containing porous particles,was immobilized in a silicone polymer sheet. This configuration also enabled to perform a simple packed-bed operation for the enzymatic oxidation of alcohol and reduction of aldehyde with a coupled-substrate coenzyme recycling in organic media.2.Immobilization of Nocardia corallina was examined for epoxidation of liquid alkenes such as 1-tetradecene,1-octene and styrene to produce corresponding epoxides in the presence of n-hexadecane as the organic solvent. The silicone polymer in which droplets of the cell suspension were finely dispersed,was the most suitable material retaining high epoxide productivity. In o … More rder to change hydrophobicity-hydrophilicity balance of the support over a wide range,it was effective to immobilize the cells in a mixed matrix composed of silicone polymer and calcium alginate gel. In the support containing excess silicone over 60%,fine particles of the alginate were dispersed in the continuous silicone phase,and in the case of alginate over 40% the phase configuration was inversed. The optimum composition of the mixed matrix was 80-90% silicone+20-10% alginate for the production of 1,2-epoxytetradecane,40-50% silicone+ 60-50% alginate for 1,2-epoxyoctane,and almost 0% silicone+100% alginate for styrene oxide. This significant change of the optimum composition was closely related to the degree of substrate inhibition. Such an optimized composite-immobilized cells offered the epoxide productivity superior to the free cells in the two-liquid phase after a longer period of batch operation,and enabled to perform a relatively simple operation of a continuous three-phase bioreactor system. Less