Relationship between membrane lipids and alcohol fermentation in Zymomonas mobilis

运动发酵单胞菌膜脂与酒精发酵的关系

• 批准号: 02660112
• 负责人: TAHARA Yasutaka
• 金额: $ 1.28万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660112/
• 关键词: Zymomonas mobilis; Glucuronic Acid-ceramide; Free Ceramide; Tetrahydroxybacteriohopane; Glucosamine-THBH; Ether-THBH; Ethanol-tolerance; Salt-tolerance; ホッパノイド

This study deals with relationship between membrane lipids and alcohol fermentation in Zymomonas mobilis, in which two long-chain base-containing lipids were found : one was a free ceramide and the other was a glucuronic acid-containing ceramide. Tetrahydroxybacteriohopane(THBH), a novel lipid of the organism, was thought to be a lipid more concerned with tolerance of high ethanol and a high salt concentrations.1. A glucuronic acid-containing ceramide was isolated from the extractable lipids by thin-layer chromatography. Hydrolysis of the lipid gave three compounds, a long-chain base, a fatty acid methyl ester and a sugar methyl ester. GC-MS analysis showed that the sugar methyl ester was that of glucuronic acid. The long-chain base and fatty acid methyl ester were the same those of the free ceramide. The three compounds were nearly same ratio in the molarity.2. The lipid composition of the organism was examined in the medium of various added ethanol. THBH increased in a high ethanol concentration, but the derivatives, THBHester and THBH-glycoside, decreased. The lipid composition was also examined in the medium of various KCl concentration. THBH only increased in a high KCl concentration.

研究了运动发酵单胞菌（Zymomonasmobilis）膜脂与酒精发酵的关系，发现其中含有两种长链含碱基的脂质：一种是游离神经酰胺，另一种是含葡萄糖醛酸的神经酰胺。四羟基菌藿烷（Tetrahydroxybacteriohopane，THBH）是一种新的生物体脂质，被认为是一种与高浓度乙醇和高浓度盐耐受性有关的脂质.通过薄层色谱法从可提取的脂质中分离出含葡萄糖醛酸的神经酰胺。脂质的水解得到三种化合物：长链碱、脂肪酸甲酯和糖甲酯。GC-MS分析表明糖甲酯为葡萄糖醛酸甲酯。长链碱和脂肪酸甲酯与游离神经酰胺相同。三种化合物的摩尔比基本相同.在添加不同乙醇的培养基中检测了该生物体的脂质组成。THBH在高乙醇浓度下增加，但衍生物THBHester和THBH-糖苷减少。在不同KCl浓度的培养基中，也检查了脂质组成。THBH仅在高KCl浓度下增加。

项目成果

Y.Tahara and M.Kawazu: "Effect of ethanol concentration on hopanoids and sphingolipids of Zymomonas mobilis" Bioscience,Biotechnology,and Biochemistry. 56. (1992)

Y.Tahara 和 M.Kawazu：“乙醇浓度对运动发酵单胞菌的藿烷类和鞘脂的影响”生物科学、生物技术和生物化学。

Y.Tahara and M.Kawazu: "Effect of salt concentration on hopanoids and sphingolipids of Zymomonas mobilis" Bioscience,Biotechnology,and Biochemistry. 56. (1992)

Y.Tahara 和 M.Kawazu：“盐浓度对运动发酵单胞菌的藿烷类化合物和鞘脂的影响”生物科学、生物技术和生物化学。

Y.Tahara and M.Kawazu: "Isolation of glucuronic acidーcontaining ceramide from Zymomonas mobilis" Agricultural and Biological Chemistry. 55. (1991)

Y.Tahara 和 M.Kawazu：“从运动发酵单胞菌中分离含葡萄糖醛酸的神经酰胺”农业和生物化学 55。（1991）

Y.Tahara and M.Kawazu: "Effect of salt concentration on hopanoids and sphingolipids of Zymomonas mobilis" Agricultural and Biological Chemistry. 55. (1991)

Y.Tahara 和 M.Kawazu：“盐浓度对运动发酵单胞菌的藿烷类化合物和鞘脂的影响”农业和生物化学。

Y.Tahara and M.Kawazu: "Isolation of a glucuronic acid-containing ceramide from Zymomonas mobilis" Bioscience,Biotechnology,and Biochemistry. 56. (1992)

Y.Tahara 和 M.Kawazu：“从运动发酵单胞菌中分离含葡萄糖醛酸的神经酰胺”生物科学、生物技术和生物化学。