Studies on alcohol tolerance of Zymomonas mobilis cell envelope

运动发酵单胞菌细胞膜的酒精耐受性研究

• 批准号: 63560100
• 负责人: TAHARA Yasutaka
• 金额: $ 1.22万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63560100/
• 关键词: Ceramide; Hopanoid; Tetrahydroxybacteriohopane; Zymomonas mobilis; Ethanol tolerance; Glucose tolerance; Nystatin-resistant mutant; エタノ-ル耐性; アルコール醗酵; アルコール耐性構造; βインプロピルマレート脱水素酵遺伝子; ショットガンクーロニング; 塩基配列決定

1. A free ceramide was isolated from membrane lipids of Z. mobilis and its molecular species was shown to be N-2-palmitoyl-1,3-dihydroxyhexadecane. 2. A PE N-methyltransferase-defective mutant was isolated from Z. mobilis, which was completely defective in all the three enzyme activities dependent on PE, PMME and PDME. Two revertants derived from the mutant recovered all the three enzyme activities to nearly the same levels of the wild type strain, suggesting that in Z. mobilis the three-step methylation in the pathway would be catalyzed by a single enzyme.3. A mutant deficient in tetrahydroxybacteriohopane (THBH) biosynthesis was isolated from Z. mobilis as a nystatin-resistant mutant. Quantitative analysis showed that the THBH content was reduced to about 20 % of that of the parent strain. The mutant exhibited to be sensitive to a high glucose concentration but tolerant to a high ethanol concentration, suggesting that in Z. mobilis the THBH might be responsible for tolerant to the former.4. A THBH-increased mutant was isolated from Z. mobiles, of which the THBH content was increased to about 3 times of that of the parent strain. Quantitative analysis of THBH, glucosamine-THBH and carbocyclic pseudopentose-THBH, in THBH-defective mutant and THBH-increased mutant suggests that the side-chains of those hopanoids, tetra- hydroxypentane, trihydroxypentenyl glucosamine and trihydroxypentenyl carbocyclic pseudopentose, would be independently incorporated into the hopane skeleton in hopanoid biosynthesis.

1.从Z. mobilis的分子种类为N-2-棕榈酰-1，3-二羟基十六烷。2.从Z. mobilis，这是完全缺陷的所有三种酶的活性依赖于PE，PMME和PDME。从突变体衍生的两个回复突变体将所有三种酶活性恢复到几乎与野生型菌株相同的水平，这表明在Z. mobilis的三步甲基化反应可能由单一酶催化.从Z. mobilis作为制霉菌素抗性突变体。定量分析表明，THBH含量降低到亲株的20%左右。该突变体对高浓度的葡萄糖敏感，但对高浓度的乙醇耐受。THBH可能是mobilis对前者耐受的原因.从Z.动子中THBH含量提高到亲本的3倍左右。对THBH缺陷突变体和THBH增加突变体中THBH、葡糖胺-THBH和碳环假戊糖-THBH的定量分析表明，在类霍帕烷生物合成中，这些类霍帕烷的侧链，四羟基戊烷、三羟基戊烯基葡糖胺和三羟基戊烯基碳环假戊糖，将独立地并入霍帕烷骨架中。

项目成果

Y.Tahara: "Isolation of Zymomonas mobilis Mutant with an Increased Amount of Tetrahydroxybacteriohopane and a Possible Biosynthesis of the Side-chain of the Hopanetetrol and Its Derivatives" Agricultural and Biological Chemistry. (1990)

Y.Tahara：“四羟基细菌藿烷含量增加的运动发酵单胞菌突变体的分离以及藿烷四醇及其衍生物侧链的可能生物合成”农业和生物化学。

Y.Tahara: "Isolation of Free Ceramide from Zymomonas mobilis" Agricultural and Biological Chemistry. 54. (1990)

Y.Tahara：“从运动发酵单胞菌中分离游离神经酰胺”农业和生物化学。

Y. Tahara et al.: "Isolation of Zymomonas mobilis Mutant with an Increased Amount of Tetrahydroxybacteriohopane and a Possible Biosynthesis of the Side-chains of the Hopanetetrol and Its Derivatives" Agricultural and Biological Chemistry, 1990.

Y. Tahara 等人：“四羟基细菌藿烷含量增加的运动发酵单胞菌突变体的分离以及藿烷四醇及其衍生物侧链的可能生物合成”农业和生物化学，1990。

Y.Tahara: "Isolation of Zymomonas mobilis Mutant with an Increased Amount of Tetrahydroxybacteriohopane and a Possible Biosynthesis of the Side-Chain of the Hopanrtrtrol and Its Derivatives" Agricultural and Biological Chemistry. (1990)

Y.Tahara：“四羟基细菌藿烷含量增加的运动发酵单胞菌突变体的分离以及藿香醇侧链及其衍生物的可能生物合成”农业和生物化学。

Y.Tahara: "Isolation of free Ceramide fron Zymomonas mobilis" Agricultural and Biological Chemistry. 54. (1990)

Y.Tahara：“从运动发酵单胞菌中分离游离神经酰胺”农业和生物化学。