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Studies on the color improvement of sugi wood by use of natural pigments

利用天然色素改良杉木色泽的研究

基本信息

批准号：
02660171
负责人：
KAI Yuji
金额：
$ 1.41万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660171/
关键词：
Sugi Color Improvement Decolorization Tissue Calture Callus Pigment Production beta-sitosterol 材色抽出成分

项目摘要

(1)Classification of sugi heartwood by colorThe values of L^*, a^* and b^* in 500 samples of sugi heartwoods were measured, and were classified into 6 groups by the difference of L^* values. VIS spectra of the methanol extracts from the 6 groups of sugi woods were measured, and spectroscopic properties of "akasin"(readish sugi heartwood)and "kurosin"(dark brown sugi heartwood)were elucidated.(2)Decolorization of the dark brown heartwoof of sugiIt was found that sodiuia chlorite decolorized most effectively sugi heartwoods among various chemical treatments, and the treatment of sodium chlorite was suited for the color conditioning of sugi heartwood since the effect of decolorization increased lineally with the concentration of sodium chlorite.(3)Production of reddish pigments by the callus culture deriving from the carabium of sugi woodThe techniques were established that a callus was derived from the cambium of sugi wood, and was cultured in an agar medium. Various experiments were conducted in order to find the culture condition producing the reddish pigments in the sugi callus. There was no relationship between the color of the callus and that of the heartwood in the explant(sugi wood). The callus having reddish pigments was often observed in the cali growing slowly under the severe condition such as in the inoistureless agar medium, but the culture condition of callus producing reddish pigment were not determined precisely. Through the above experiments, several new observations were obtained on the relationship between phytohormones and callus development, and on the production of beta-sitosterol in the sugi callus.

（1）杉心材的颜色分类测定了500个杉心材样品的L^*、a^* 和B^* 值，并根据L^* 值的差异将其分为6类。测定了6种不同类型的杉材甲醇提取物的维斯光谱，并对“赤心木”和“黑心木”的光谱性质进行了分析。（2）深棕色杉心材的脱色研究结果表明，在各种化学处理中，NaCl 2对杉心材的脱色效果最好，NaCl 2处理对杉心材的脱色效果与NaCl 2浓度呈线性关系，适合于杉心材的颜色调理。（3）来自杉木腕足的愈伤组织培养产生红色色素建立了来自杉木形成层的愈伤组织在琼脂培养基中培养的技术。为了探索杉愈伤组织产生红色色素的培养条件，进行了各种试验。愈伤组织的颜色与外植体（杉材）心材的颜色之间没有关系。卡利愈伤组织在无营养盐琼脂培养基等苛刻条件下生长缓慢，常出现红色愈伤组织，但产生红色愈伤组织的培养条件尚未确定。通过上述实验，对植物激素与愈伤组织发育的关系，以及对杉愈伤组织中β-谷甾醇的产生，获得了一些新的观察结果。