权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Bio-synthesis and Function of a Novel Cross-linking Amio Acid, of Elastin Allodesmosine.

弹性蛋白别锁链素的新型交联氨基酸的生物合成和功能。

基本信息

批准号：
02660293
负责人：
SUYAMA Kyozo
金额：
$ 1.15万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660293/
关键词：
Elastin Cross-linking amino acid Allodesmosine Aldol Cross-link

项目摘要

Elastin is a connective tissue protein found in virtually every tissue and organs. Elastin is primarily synthesized during physiological development by cells as a soluble precursor, tropoelastin. Tropoelastin are exposed to the extracellular space Ydierc. - they continue to undergo chemical modifications that result uLtimately in formation of specific kinds of covalent attachments, called cross-link. Ultimately, a family of different crosslinks restilts, many members of which have now have been isolated and identified.A new pentafmotional cross-]Linking andno acid, termed allodesmosine, was isolated from bovine lige-mntum nuchae, aorta and lung elastin This was characterized by LAI, NMR and MS spectroscopy. The compound was shown by Uv and 1H-NMR to have pyridinium ring structure. Mass spectral analysis indicated a pELrent ural with a mass of 655. We postulated that it arose by condensation of a redurw aldol condensation products via aldol crogs-liriking amino acid, aldosine, which is novel amino acid isolated AM identified in this project by our laboratory.In our model reaction, we employed the aldol corxiensation products of propanal, 2methyl-2-pentenal(MP), as aldehydes, and n-butylamine(l)and lysine(2)as primary amities. The reaction of MP and I and 2 was carried out under the neutral condition at room temperature. Hofmann degradation(160-170 ﾟC for 10min)of water soltibl. e reactanlq gave various pyridines, 2-ethyl-, 4-etityl- and 2-(l-methyl-l-biitenyl)-3, 5-dimethylpyridine with other nuidetitLfied pyridines. From the reaction products of MP and 2, 1-alpha-aminoadipicaid 2-(l-mtyl-l-buLe-nyl.)-3, 5-dimetylpyridinium which was formffl by the condensation of 2 moles of MP and epsilon-amnino group of lysine was separated and identified. A highperforwmce liquid chromatgraphic procedxjlre for the determination of allodesmosine with other cross-links has been developed.

弹性蛋白是一种结缔组织蛋白，几乎存在于每个组织和器官中。弹性蛋白主要在生理发育期间由细胞作为可溶性前体原弹性蛋白合成。原弹性蛋白暴露于细胞外空间Ydierc。- 它们继续进行化学修饰，最终导致形成特定种类的共价连接，称为交联。从牛颈韧带、主动脉和肺弹性蛋白中分离得到一个新的五氟甲基交联氨基酸，命名为异锁链素。通过紫外光谱和核磁共振氢谱证实了该化合物具有吡啶鎓环结构。质谱分析表明是一个质量为655的质子。本实验以丙醛的羟醛缩合产物2-甲基-2-戊烯醛（MP）为醛类，正丁胺（1）和赖氨酸（2）为伯胺，通过羟醛缩合反应合成了一种新的氨基酸-醛辛（Aldosine）。MP与I和2的反应在室温中性条件下进行。Hofmann降解（160-170 ℃，10 min）水溶性。反应得到各种吡啶，2-乙基-，4-乙基-和2-（1-甲基-1-联烯基）-3，5-二甲基吡啶以及其它未取代的吡啶。从MP和2，1-α-氨基己二酸2-（1-甲基-1-丁基）-酯的反应产物，分离并鉴定了2摩尔MP与赖氨酸的ε-氨基缩合生成的3，5-二甲基吡啶。本文建立了一种高效液相色谱法测定异锁链素的新方法。