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Quantitative analysis of cross-linking amino acid of elastin in aorta and pulmonary artery, associated with aortic aneurysm and pulmonary hypertension, respectively

主动脉和肺动脉弹性蛋白交联氨基酸的定量分析，分别与主动脉瘤和肺动脉高压相关

基本信息

批准号：
09670175
负责人：
SAWAI Takashi
金额：
$ 2.05万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Objective : To elucidate the alteration of various cross-linking amino acids in dissecting aortic aneurysm and pulmonary hypertension, we analyzed the large arteries of elastic type by a new method using high-performance liquid chromatography (HPLC), furthermore, examined the expression of several matrix metalloproteinases (MMPs) related to elastin degradation by immunohistochemical method.Materials : used for HPLC were all taken from autopsy cases, and aorta with aneurysm for IHC was obtained at operation.Analytical method : HPLC method was followed by Suyama's reports published somewhere before. He is one of our collaborators.Results :1. Quantity of linking of amino acid in aorta and dissecting aneurysm, and expression of MMP related to elastin degradation(1) Desmosine and isodesmosine of human aorta were decreased as aging process, furthermore, the ratio of oxodesmosine and isooxodesmosine to desmosine and isodesmosine were also decreased, which reveals the weakness of aortic wall a … More partly depends on the lowering the volume of cross-linking of amino acids.(2) Among several proteolytic enzymes, related to elastin degradation, MMP-2 and MMP-9 were immunohistochemically demonstrated in dissecting aneurysm.2. Quantity of cross-linking of amino acid in pulmonary artery in aging process and pulmonary hypertensionIn none of the cases did the amount of desmosine contained in the walls of the pulmonary artery prove to be less than the amount of isodesmosine. The PA walls were shown to contain smaller amounts of desmosine and isodesmosine than were those of aorta. No. significant correlation proved to exist between age and the volume of desmosine and isodesmosine. In Eisenmenger' syndrome and mixed connective tissue disease (MCTD), with pulmonary hypertension, the pulmonary arterial walls showed decrease of desmosine and isodesmosine. We are now studying the immunohistochemical analysis of desmosine in aorta and pulmonary artery, and try to compare with the change of collagen fibers. Less

目的：采用高效液相色谱（HPLC）分析弹性型大动脉组织中各种交联氨基酸的变化，并采用免疫组化方法检测与弹性蛋白降解相关的基质金属蛋白酶（MMPs）的表达。材料：HPLC所用材料均取自尸检病例，手术取动脉瘤主动脉进行IHC。分析方法：采用高效液相色谱法（HPLC）。他是我们的合作者之一。结果:1。主动脉和夹层动脉瘤中氨基酸的连接量以及与弹性蛋白降解相关的MMP的表达(1)人主动脉的desmose和isodesmose随着年龄的增长而减少，并且oxodesmose和isoxodesmose与desmose和isodesmose的比值也减少，这表明主动脉壁的薄弱，更多的是由于氨基酸交联量的减少。(2)在与弹性蛋白降解有关的几种蛋白水解酶中，MMP-2和MMP-9在夹层动脉瘤中被免疫组织化学证实。衰老过程中肺动脉内氨基酸交联量与肺动脉高压的关系没有一例肺动脉壁上含有的聚氨基葡萄糖少于异聚氨基葡萄糖。与主动脉壁相比，PA壁含有较少的桥糖素和异桥糖素。否。年龄与桥mosine和异桥mosine的含量存在显著相关性。艾森曼格综合征和混合性结缔组织病（MCTD）合并肺动脉高压时，肺动脉壁上的桥糖素和异桥糖素均降低。我们目前正在研究对主动脉和肺动脉中氨基葡萄糖的免疫组化分析，并试图与胶原纤维的变化进行比较。少