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Regulation of Nervous Activity by Endogenous Prostaniods

内源性前列腺素对神经活动的调节

基本信息

批准号：
02660315
负责人：
YAGASAKI Osamu
金额：
$ 1.09万
依托单位：
University of Osaka Prefecture
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660315/
关键词：
Myenteric plexus Acetylcholine Release prostanoids Cyclic AMP Arachidonic Acid Guinea-pig Small Intestine Transmural Stimulation Nicotinic Stimulation アラキドン酸経壁刺激ニコチン刺激プロスタグランジン伝達物質放出 Vas deferens Myenteric Plexus ノルアドレナリン

项目摘要

Effects on the contraction of vas deferens induced by nerve stimulation : In guinea pig vas deferens, both pre- and post-ganglionic stimulations of hypogastric nerve induced a biphasic contraction consisting of a rapig transient phas and delayed tonic Phase. Indomethacin partly inhibited the contraction induced by pre-ganglionic stimulation, but not the responses by post-ganglionic stimulation. The indomethacin inhibition was reversed by PGE_2- PGE_2 also delayed the onset of phasic contraction and increased the height of both phasic and tonic contractions, and significantly enhanced the contraction induced by authentic ATP and norepinephrine. The contraction induced by pre-ganglionic stimulation was inhibited by mepacrine and PGE_2 receptor antagonist, SC19220. PGE_2 and arachidonic acid reversed these inhibition. The findings suggest that endogenous PGE_2 is important in neurotransmission in the plevic ganglion of guinea pig.Effects on the release of ACh from the myenteric plexus of … More guinea pig ileum : The amount of ACh released in response to nicotine or substance P was depressed to about 40% of control by indomethacin, whereas that induced by 5-HT or electrical field stimulaltion(EFS)was not affected. The inhibitory effect of indomethacin were reversed by PGE_2. Mepacrine, an inhibitor of pbospbolipase A_2, depressed the release of ACh by nicotine and substance P to the same extent as indomethacin. OP-41483, an analogue of PGI_2, recovered inhibited nicotine-induced release more effectively than PGE_2. This effect was abolished by H-89, an A-kinase inhibitor. PGD_2, PGF_<2alpha> and TXA_2 analogue were ineffective. PGI_2 was released during incubation of preparation in the amount as much as 5 times of PGE_2. These results indicate that both endogenous PGI_2 and PGE_2 regulate the excitability of cholinergic neurons in the myenteric plexus and that the effects are mediated by the activation of intracellular cyclic AMP system. The ACh release by EFS was inhibited by 5-lipooxygenase inhibitors but not by 12-lipoxygenase inhibitor. The inhibitory effects were reversed by arachidonic acid, 5-HETE, LTD_4 and LTE_4, but not by LTB_4 and LTC_4, indicating the regulation5-lipoxygenase metavolites on efs induced ach release.In conclusion, many prostanoias contribute for maintaing the activity of the myeyerixc plecus of guinea pig iluem, but the kind of prostanoid concerned may diffe with different stimuli applied to nerve. Less

神经刺激对输精管收缩的影响：在豚鼠输精管中，节前和节后刺激腹下神经均可引起由快速短暂期和延迟性紧张期组成的双相收缩。吲哚美辛部分抑制节前刺激引起的收缩，但不能抑制节后刺激引起的收缩反应。前列腺素E_2-前列腺素E_2可逆转吲哚美辛的抑制作用，延缓时相收缩的开始时间，增加时相收缩和紧张性收缩的高度，并显著增强正品三磷酸腺苷和去甲肾上腺素引起的收缩。美帕林和PGE_2受体拮抗剂SC19220可抑制节前刺激引起的收缩。前列腺素E_2和花生四烯酸可逆转这种抑制作用。结果提示，内源性前列腺素E_2在豚鼠…肌间神经节的神经传递中起重要作用。对ACh释放的影响更多的豚鼠回肠：吲哚美辛可使尼古丁或P物质引起的ACh释放量减少到对照组的40%左右，而5-羟色胺或电场刺激(EFS)引起的ACh释放量不受影响。前列腺素E_2可逆转吲哚美辛的抑制作用。磷脂酶A_2的抑制剂美帕克林对烟碱和P物质释放ACh的抑制作用与吲哚美辛相同。前列腺素I_2的类似物OP-41483恢复后比前列腺素E_2更有效地抑制尼古丁诱导的释放，这一作用可被A-激酶抑制剂H-89取消。前列腺素D_2、前列腺素F_2α和血栓素A_2类似物无效。结果表明，内源性前列腺素I_2和前列腺素E_2均可调节肌间神经丛胆碱能神经元的兴奋性，这种作用是通过激活细胞内环磷酸腺苷系统实现的。EFS释放ACh可被5-脂氧合酶抑制剂抑制，但不能被12-脂氧合酶抑制剂抑制。花生四烯酸、5-HETE、LTD_4和LTE_4可逆转这种抑制作用，而LTB_4和LTC_4则不能，这说明5-脂氧合酶代谢物对EFS诱导的ACh释放有调节作用。总之，许多前列腺素有助于维持豚鼠肠胃肌丛的活动，但所涉及的前列腺素可能因神经刺激而不同。较少