Expression of period repeat sequence consisting in Drosopila clock gene in the rat brain : Insitu hybridization study.

果蝇时钟基因组成的周期重复序列在大鼠脑中的表达：原位杂交研究。

• 批准号: 02670028
• 负责人: TOKUMAGA Akira
• 金额: $ 0.26万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670028/
• 关键词: Clock Gene; Suprachiasmatic Nucleus; In Situ Hybridization; Circadian Rhythm; Rat Brain

The clock gene of Drosophila containing a hexamer repeat sequence(ACAGGC)_n (period, per, repeat)is reported to be concerned in ther circadian rhythm. Young and his co-workers(1987)succeeded to clone a mouse genomic DNA containing the per repeat(cp2.2). In this study, temporal and spacial expression of CP2.2F and RB15 was examined in the adult rat brain by in situ hybridization. The former is a family of genes detected by using cp2.2 as a probe, and the latter is a gene corresponding to pRB15, one of the 11 cDNA recombinants homologous to cp2.2 from the rat brain cDNA libraly. Both genes were expressed in almost all neurons. However, it was in the suprachiasmatic nucleus that fluctuation to the genes under the light-dark cycle was apparently observed ; an intensive hybridization signal in the middle of the day and a weak in the middle of the night(p<0.01). On the other hand, the signal stayed relatively constant in other brain regions. A weak expression of both genes(CP2.2F and RB15)was detected in some glial and ependymal cells in the day but only a few in the night.The present findings suggest that CP2.2F and RB15 may contribute to the circadian rhythm in the rat nervous system.

果蝇的时钟基因含有一个六聚体重复序列（ACAGGC）_n（period，per，repeat），与昼夜节律有关。Young和他的同事（1987）成功地克隆了含有每个重复序列（cp2.2）的小鼠基因组DNA。在这项研究中，时间和空间表达的CP 2.2F和RB15在成年大鼠脑原位杂交检查。前者是以cp2.2为探针检测到的一个基因家族，后者是从大鼠脑cDNA文库中克隆的11个与cp2.2同源的cDNA重组体之一pRB 15的对应基因。这两种基因在几乎所有神经元中都有表达。而在视交叉上核，则出现了明显的光暗周期波动，在白天杂交信号较强，而在夜间杂交信号较弱（p<0.01）。另一方面，信号在其他大脑区域保持相对稳定。CP2.2F和RB 15基因在大鼠神经系统的昼夜节律中有微弱的表达，但在夜间仅有少数表达。

项目成果

Ishida, N., Noji, S., Ono, K., Koyama, E., Nohno, T., Taniguchi, S., Tokunaga, A., Fujii, T. and Mitsui, Y: "Diurnal regulation of per repeat mRNA in the suprachiasmatic nucleus in rat brain." Neuroschi. Letters. 122. 113-116 (1991)

Ishida, N.、Noji, S.、Ono, K.、Koyama, E.、Nohno, T.、Taniguchi, S.、Tokunaga, A.、Fujii, T. 和 Mitsui, Y：“每次重复的昼夜调节

Shibata, N.: "Expression of period (per) repeat sequence consisting in Drosophila clock gene in the rat. (In Japanese)" Okayama-Igakukai-Zasshi. 104. (1992)

Shibata, N.：“果蝇时钟基因在大鼠中的周期（每个）重复序列的表达。（日语）”Okayama-Igakukai-Zasshi。

Shibata, N., Noji, S., Ishida, M., Ono, K., Taniguchi, S., Mitsui, Y., Ohmoto, T., and Tokunaga, A.: "Expression of period repeat mRNA in the suprachiasmatic nucleus of the rat brain." Neurosci. Research. Suppl. 16. S152 (1991)

Shibata, N.、Noji, S.、Ishida, M.、Ono, K.、Taniguchi, S.、Mitsui, Y.、Ohmoto, T. 和 Tokunaga, A.：“视交叉上周期重复 mRNA 的表达

柴田 直樹: "ショウジョウバエ時計遺伝子に存在するperiod(per)repeatを持つ遺伝子のラット脳内発現" 岡山医学会雑誌. 104. (1992)

Naoki Shibata：“大鼠大脑果蝇时钟基因中周期（每）重复基因的表达”冈山医学会杂志 104。（1992）

Ishida,N.,et al.: "Diurnal regulation of per repeat mRNA in the suprachiasmatic nucleus in rat brain." Neuroscience Letters. 122. 113-116 (1991)

Ishida,N.,et al.：“大鼠大脑视交叉上核中每次重复 mRNA 的昼夜调节。”