虚血性脳浮腫病巣における血管外漏出血骨蛋白質の分布と定量的画像化

缺血性脑水肿病灶外渗出血性骨蛋白的分布及定量成像

• 批准号: 02670146
• 负责人: 黒岩 俊彦
• 金额: $ 0.9万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670146/
• 关键词: Brain edema; cold lesion; Evans blue; albumin

本年度は虚血性脳浮腫の主体を占める血管源性脳浮腫の際の血管外漏出血清蛋白質の分布の定量的画像化を試みた.実験には成猫を用い、麻酔下・手術用顕微鏡下に頭頂部大脳皮質を露出し凍結性脳損傷を作成した.血清アルブミンを漏出血清蛋白の指標として用いEvans blue combined serum albumin濃度をPatlakらの持続注入法(1976 J Appl Physiol)により実験期間中一定に保ち、病変作成後6時間目に動物を屠殺した(黒岩).そして直ちに血管内トレ-サ-を生食の潅流でwash outした後、摘出脳を中大脳動脈支配領域で冠状断し一定照度下でEvans blue濃度のstandardと共に写真撮影しdensitometerにより定量化した.また血中Evans bluealbumin complexのspecific activityを求め大脳割面上各部位の血管外漏出アルブミン量(mg/g.brain)を定量的に画像表示した.そして画像に対応する残存した脳の鏡面ブロックより病単各部位の浮腫液量を組織比重法により求め、浮腫病単各部位における増過水分量、浮腫液アルブミン含量を求めた(黒岩、桶田).さらに血管外漏出アルブミン定量に用いたブロックはその後浸潤固定し、組織像との対比を行なった.以上の結果、病巣に於ける脳浮腫液の分布の定量的画像化は血管源性脳浮腫の際は可能であることが分かった.そして血管外漏出血清アルブミン量と浮腫に程度よく相関すること、凍結脳損傷の際の浮腫液の分布には一定のパタ-ンが有り、各脳回の直下の白質に比べて脳室周囲の白質(Centrum semiovale)の方に浮腫液はより多く貯留する傾向の有ることが判明した.

This year, the main body of hematuria edema is accounted for. The distribution of serum protein in the extravascular leakage at the time of vasogenic edema is quantitatively characterized. For surgery, the large cortex of the top of the head is exposed under a microscope. As an indicator of serum albumin leakage, the Evans blue combined serum albumin concentration was used by Patlak's sustained injection method (1976 J Appl Physiol) to ensure that all animals were kept safe during the experiment and slaughtered 6 hours after the onset of the disease (Black Rock). The concentration of Evans blue in the coronary artery dominated by the coronary artery under a certain illumination standard was quantified by densitometer. The specific activity of Evans bluealbumin complex in the blood was determined quantitatively by the quantitative quantitative analysis of extravascular leakage (mg/g.brain) at various sites on the large cut surface. The amount of edema fluid in each part of the disease was determined by histogravimetric method. The amount of water increased in each part of the disease and the amount of edema fluid were determined by histogravimetric method (black rock, barrel field). In addition, the quantitative analysis of extravascular leakage was carried out by means of tissue imaging and tissue fixation. The above results show that the quantitative distribution of edema fluid in patients with vascular edema is possible. There is a correlation between the amount of extravascular leakage of serum and the degree of edema. The distribution of edema fluid during freezing injury has a certain degree of correlation. The straight line under each loop has a tendency to store more edema fluid than the straight line around the ventricle.

项目成果

Toshihiko KUBOIWA,Riki Okeda et al: "Brain Edema VIII" H.J.Reulen,A Baethmann,et al Springe Verlag, 420 (1991)

Toshihiko KUBOIWA、Riki Okeda 等人：“脑水肿 VIII” H.J.Reulen、A Baethmann 等人 Springe Verlag，420 (1991)