虚血性脳浮腫病巣における血管外漏出血骨蛋白質の分布と定量的画像化

缺血性脑水肿病灶外渗出血性骨蛋白的分布及定量成像

• 批准号: 02670146
• 负责人: 黒岩 俊彦
• 金额: $ 0.9万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670146/
• 关键词: Brain edema; cold lesion; Evans blue; albumin

本年度は虚血性脳浮腫の主体を占める血管源性脳浮腫の際の血管外漏出血清蛋白質の分布の定量的画像化を試みた.実験には成猫を用い、麻酔下・手術用顕微鏡下に頭頂部大脳皮質を露出し凍結性脳損傷を作成した.血清アルブミンを漏出血清蛋白の指標として用いEvans blue combined serum albumin濃度をPatlakらの持続注入法(1976 J Appl Physiol)により実験期間中一定に保ち、病変作成後6時間目に動物を屠殺した(黒岩).そして直ちに血管内トレ-サ-を生食の潅流でwash outした後、摘出脳を中大脳動脈支配領域で冠状断し一定照度下でEvans blue濃度のstandardと共に写真撮影しdensitometerにより定量化した.また血中Evans bluealbumin complexのspecific activityを求め大脳割面上各部位の血管外漏出アルブミン量(mg/g.brain)を定量的に画像表示した.そして画像に対応する残存した脳の鏡面ブロックより病単各部位の浮腫液量を組織比重法により求め、浮腫病単各部位における増過水分量、浮腫液アルブミン含量を求めた(黒岩、桶田).さらに血管外漏出アルブミン定量に用いたブロックはその後浸潤固定し、組織像との対比を行なった.以上の結果、病巣に於ける脳浮腫液の分布の定量的画像化は血管源性脳浮腫の際は可能であることが分かった.そして血管外漏出血清アルブミン量と浮腫に程度よく相関すること、凍結脳損傷の際の浮腫液の分布には一定のパタ-ンが有り、各脳回の直下の白質に比べて脳室周囲の白質(Centrum semiovale)の方に浮腫液はより多く貯留する傾向の有ることが判明した.

This year, the "virtual blood" floating body accounts for the quantitative imaging test of the distribution of extravascular serum protein in the vascular float. The skin of the adult cat and the anaesthesia were made with the skin of the large skin of the cat under the micro-microscope. The leakage of serum albumin from the serum was determined by the method of Evans blue combined serum albumin concentration and Patlak injection (1976 J Appl Physiol). The animals were killed 6 hours after the onset of the disease. After the intravascular injection of raw food was carried out directly, the wash out was removed, and the control field was cut off under certain illuminance of Evans blue, standard, and densitometer pictures. The quantitative "portrait representation" (mg/g.brain) of extravascular leakage (mg/g.brain) in each part of the cut surface was calculated for the Evans bluealbumin complex in the blood. The picture shows that the water content of each part of the disease is calculated by tissue specific gravity method, the amount of water in each part of the disease is measured by tissue specific gravity method, and the content of the liquid is measured by tissue specific gravity method. The quantity of extravascular leakage was measured by immersion fixation and tissue image analysis. As a result of the above results, the disease may be affected by quantitative imaging of the distribution of the floating fluid. The amount of serum leaking out of the blood vessels was measured. The concentration of the fluid was measured. The results showed that the distribution of the liquid floatation was more sensitive than that of the periphery of the room (Centrum semiovale).

项目成果

Toshihiko KUBOIWA,Riki Okeda et al: "Brain Edema VIII" H.J.Reulen,A Baethmann,et al Springe Verlag, 420 (1991)

Toshihiko KUBOIWA、Riki Okeda 等人：“脑水肿 VIII” H.J.Reulen、A Baethmann 等人 Springe Verlag，420 (1991)