Immunohistochemical study on atrial natriuretic peptide in the ventricle of hearts of infants and children.

婴儿和儿童心室心房钠尿肽的免疫组织化学研究。

• 批准号: 02670142
• 负责人: NISHIKAWA Toshio
• 金额: $ 0.9万
• 依托单位: Tokyo Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670142/
• 关键词: Natriuretic peptide; Ventricular myocyte; Heart muscle disease in infants; Immunohistochemistry; Ultrastructure; Immunoelectron microscopy; Specific granule; Myocyte stress; 心房利尿ペプチド; 心内膜線維弾性症; 心筋症; 免疫組織化学; 酵素抗体法; 左心室容積; 心筋細胞横径

In this study, the presence and distribution of human atrial natriuretic peptide (ANP) were i. iivestigated iijimtinoliistoclieniically in tlie ventricle of the hearts of infantsaiid children. ANP-immunoi, eactive myocytes were cleai, ly deinoiisti, at. e(i iii tlie veiiti, icle of liearts wi L. Ii endocardial fibi, oel-astosis and dilated cardioinyopat, iiy. The distri. btit. i. oii of ANP-positive inyocytes was most frequent iii the inner otie-third of tlie left ventricle. No ANP'immunoreactivity was detected in any lieart in cases with congei-iital heart disease or noncardiac disease. The left ventricular volume of liearts with ANP-positive myocytes was larger than that witli ANP-negetive myocytes. The inean diameter of ANP-positive niyocytes was greater tilail that of ANP-negative myocytes. Iminunohistochemical study was also carried out in the biopsied ventricular myocardium of endocardial fibroelastosis. The frequency of ANP-positive myocytes in the biopsied specimens significantly correlated with left ventricular end-diastolic volume (r=0.735) and inversely correlated with ejection fraction (r=0.730). It was also correlated with histopathologic severity index (inyocyte hypertrophy, degeneration and interstitial fibrosis) (r=0.762). Ultrastructural examination confirmed the presence of specific electrondense granules of 250-400 nm in diameter in the ventricular myocytes. Immunoelectron microscopy demonstrated the presence of ANP-labeling colloidal gold particles in the specific granules. It was also demonstrated that brain natriuretic peptide was colocalized with ANP in some of the granules. The results of this study suggest that ANP expression in the ventricle of heart muscle disease in infancy and childhood is related to excess of wall stresses and of myocyte stresses.

本文观察了人心钠素(ANP)在婴幼儿心脏的存在和分布。ANP免疫阳性，活跃的心肌细胞清晰可见。心内膜超声心动图，心内膜肥厚，心内膜扩张。迪斯尼。Bitt.心钠素阳性的心肌细胞以左心室内侧1/3最多见。在先天性心脏病和非心脏病患者的血清中均未检测到ANP免疫反应。心钠素阳性心肌细胞的左心室容量大于心钠素阴性心肌细胞者。ANP阳性的心肌细胞直径大于ANP阴性的心肌细胞。对心内膜弹力纤维增生症的心肌活检标本进行免疫组织化学研究。心钠素阳性心肌细胞的出现频率与左心室舒张末容积呈显著正相关(r=0.735)，与射血分数呈负相关(r=0.730)。与病理严重程度指数(细胞肥大、变性、间质纤维化)呈正相关(r=0.762)。超微结构检查证实，心肌细胞内存在直径250-400 nm的特殊电子致密颗粒。免疫电子显微镜显示ANP标记的胶体金颗粒存在于特定的颗粒中。脑利钠肽与心钠素共存于部分颗粒内。本研究结果提示，心钠素在婴幼儿和儿童期心肌病患者的心室中的表达与室壁应力过大和心肌细胞应力过大有关。

项目成果

Sumio Nakamura,Mitsuhide Naruse,Kiyoko Naruse,Toshio Nishikawa,et al.: "Atrial natriuretic peptide and brain natriuretic peptide coexist in the secretory granules of human cardiac myocytes." American Journal of Hypertension. 4. 909-912 (1991)

Sumio Nakamura、Mitsuhide Naruse、Kiyoko Naruse、Toshio Nishikawa等人：“心房钠尿肽和脑钠尿肽共存于人心肌细胞的分泌颗粒中。”

Toshio Nishikawa,Takeshi Kasajima,Takao Kanai: "Potentiating effects of forskolin on the cardiovascular teratoーgenicity of ephedrine in chick embryos." Toxicology Letters. 56. 145-150 (1991)

Toshio Nishikawa、Takeshi Kasajima、Takao Kanai：“毛喉素对鸡胚胎中麻黄碱的心血管致畸性的增强作用。” 56. 145-150 (1991)。

Toshio Nishikawa, Morie Sekiguchi, Atsuyoshi Takao, Masahiko Ando, Michiaki Hiroe, Shinichiro Morimoto, Takeshi Kasajima: "Histopathological assessment of endomyocardial biopsy in children : I. Semiquantitative study on the hypertrophy of cardiac myocytes

Toshio Nishikawa、Morie Sekiguchi、Atsuyoshi Takao、Masahiko Ando、Michiaki Hiroe、Shinichiro Morimoto、Takeshi Kasajima：“儿童心内膜心肌活检的组织病理学评估：I.心肌细胞肥大的半定量研究

西川 俊郎,笠島 武,長尾 博明: "ヒトの心筋の発達による変化。" Japan Jornal of Electrocardiology. 10(sup 1). 119-122 (1990)

Toshiro Nishikawa、Takeshi Kasashima、Hiroaki Nagao：“人类心肌的发育变化。”日本心电学杂志 10（sup 1）（1990 年）。

Shinichiro Imamura, Rumiko Matsuoka, Eriko Hiratsuka, Misa Kimura, Toshio Nakanishi, Toshio Nishikawa, Yoshiyuki Furutani, Atsuyoshi Takao: "Adaptational changes of MHC gene expression and isozyme transition in cardiac overloading." American J. Physiology

Shinichiro Imamura、Rumiko Matsuoka、Eriko Hiratsuka、Misa Kimura、Toshio Nakanishi、Toshio Nishikawa、Yoshiyuki Furutani、Atsuyoshi Takao：“心脏超负荷时 MHC 基因表达和同工酶转变的适应性变化。”