Studies on control of ciliary activity in triton-extracted models of schistosome miracidia

Triton提取的血吸虫毛毛虫模型中纤毛活动控制的研究

• 批准号: 02670170
• 负责人: YOSHIKI Aoki
• 金额: $ 1.54万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670170/
• 关键词: Schistosome; Miracidia; Ciliary activity; Triton-extracted model; Osmotic pressure; ミラジジウム

The study was designed to explore the mechanism(s) of control of activity of cilia which are present all over the surface of schistosome miracidia. The hatched miracidia and miracidia enclosed within the eggshell of Schistosoma mansoni were used in this study.Scanning electron microscopy revealed that the body is covered with numerous cilia, 3 different cilia are present, and the major cilia are 8.5 mum in length and 0.25 mum in width, which distribute all over the surface except the anterior tip of the body.The swimming speed of miracidia was measured in NaCl, KCl and sucrase solution. The swimming speed of miracidia is a function of the concentration of NaCl, KCl and suorose. These results indicate that beating frequency of miracidial cilia is depend on the osmotic presure.Control of ciliary activities by exterally applied reagents was studied in triton-extracted model. The extraction and reactivating solution used for triton-extracted model of sperm(Gibbons and Gibbons, 1982)was used for schistosome miracidia. Interestingly enough the beating frequency of cilia is function of NaCl concentation. The beating frequency of cilia is also depend on ATP and Mg++ concentration and pH of medium. In a standard reactivating medium (4mM ATP,lmM MgSO4, pH8.1), the models swim at 1/3 of swimming speed of the live miracidia in water(2m/sec). Ca++ activated partially the ciliary beating, but did not cause the reversed direction of beating. Other divalent cations did not activate the ciliary beating. VO3 caused the inhibition of ciliary beating.Ca++ ionophore and phorbol esters activated the cilial beating of miracidia enclosed within the eggshell. W-7, H-7 and calcium channel blocker inhibited the cilial beating of miracidia enclosed within the eggshell.Pharmacological studies on control mechanism of ciliary activities is now in progress.

本研究旨在探讨血吸虫纤毛活性的控制机制，纤毛分布在血吸虫表面。本研究以曼氏血吸虫孵化的小虫和包在卵壳内的小虫为研究对象。扫描电镜显示，体表被大量纤毛覆盖，存在3种不同的纤毛，主纤毛长8.5 mm，宽0.25 mm，除体表前端外，其余部位均有分布。测定了miracidia在NaCl、KCl和蔗糖溶液中的游动速度。miracidida的游动速度是NaCl、KCl和蔗糖浓度的函数。这些结果表明，微纤毛的跳动频率与渗透压有关。在triton萃取模型中，研究了外用试剂对纤毛活性的控制。用于triton提取精子模型的提取和再激活液（Gibbons and Gibbons, 1982）用于微型血吸虫。有趣的是，纤毛的跳动频率是NaCl浓度的函数。纤毛的跳动频率还与ATP、Mg++浓度和培养基pH有关。在标准的再活化培养基（4mM ATP,lmM MgSO4, pH8.1）中，模型以活的miracidia在水中的1/3游泳速度（2m/sec）游泳。Ca++部分激活了纤毛搏动，但没有引起搏动方向的逆转。其他二价阳离子不激活纤毛搏动。VO3对纤毛跳动有抑制作用。钙离子离子和磷酯激活了封闭在蛋壳内的微藻的纤毛跳动。W-7、H-7和钙通道阻滞剂抑制了封闭在蛋壳内的微藻的纤毛跳动。对纤毛活性调控机制的药理研究正在进行中。

项目成果

Kenichiro Matsumura: "Schistosoma mansoni:Possible Involvement of Protein Kinase C in Linoleic acidーinduced Proteolytic Enzyme Release from Cercariae" Experimental Parasitology.

Kenichiro Matsumura：“曼氏血吸虫：蛋白激酶 C 可能参与亚油酸诱导的尾蚴蛋白水解酶释放”实验寄生虫学。

K.Matsumura,Y.Mitsui,K.Sato,M.Sakamoto and Y.Aoki: "Schistosoma mansoni:Possible involvement of protein kinase Cinlinoleic acidーinduced proteolytic enzyme release from cercariae" Experimental Parasitology. 72. 311-320 (1991)

K. Matsumura、Y. Mitsui、K. Sato、M. Sakamoto 和 Y. Aoki：“曼氏血吸虫：可能涉及蛋白激酶辛油酸诱导的尾蚴蛋白水解酶释放”实验寄生虫学 72. 311-320 (1991)。