Image analysis of organ disorder caused by alcohol

1.方法将中法尸检中取出的肝脏和全脑残片用10%主液固定。阿赞和油红0染色后，取肝脏进行显微照相。在大脑额叶水平上，分别拍摄了穹隆体部、乳头体和顶枕沟水平的脑部断层照片。方法：1.应用图像分析系统测定大鼠肝纤维化或脂肪变性程度，测量脑灰质和白质面积。结果5例正常人纤维成分/总面积为0.72~3.91%(平均2.55)，7例脂肪肝为1.68~4.63%(3.36)，9例脂肪肝合并肝硬变为6.44~30.06%(13.89)，6例肝硬变为7.34~28.76%(18.11)。5例脂肪肝脂肪成分/总面积为9.29~49.62%(24.89)。额叶灰质/总面积平均值为0.552。顶叶0.501例，颞叶0.641例，枕叶0.735例。额叶白质/总面积的平均值为0.448。顶叶为0.499，颞叶为0.350，枕叶为0.246。在6例酗酒者脑中，额叶灰质/总面积的平均值为0.552。顶叶0.535例，颞叶0.690例，枕叶0.672例。额叶白质/总面积的平均值为0.478。其中顶叶0.465例，颞叶0.672例，枕叶0.328例。酗酒组额叶、枕叶灰质萎缩，顶叶、颞叶白质萎缩。

1. MethodsFragments of liver and total brain which were taken out in the medio-legal autopsy were fixed with 10% formain solution. After Azan and oil-red 0 stains were carried out, microscopic photographs of liver were taken. Photographs of cerebral frontal sections at the level of Genu of corpus callosum, Mammillaly bodies and Parieto-occipital sulci were done. Using with image analysing system, degree of liver fibrosis or adiposis were determined and areas of cerebral gray seatter and white matter were measured.2. ResultsFiber component/total area was 0.72-3.91%(average 2.55)in the 5 normal cases, 1.68-4.63%(3.36)in the 7 cases of fatty liver, 6.44-30.06%(13.89)in the 9 cases of fatty liver with liver cirrhosis and 7.34-28.76%(18.11)in the 6 cases of liver cirrhosis.Fat componemt/total area were 9.29-49.62%(24.89)in the 5 cases of fatty liver.In the 15 normal cases, average of cerebral gray matter/total area was 0.552 in the frontal lobe. The one in the parietal lobe was 0.501, in the temporal lobe 0.641 and in the occipital lobe 0.735. Average of white matter/total area was 0.448 in the frontal lobe. The one in the parietal lobe was 0.499, in the temporal lobe 0.350 and in the occipital lobe 0.246.In the 6 brains of alcoholics, average of gray matter/total area was 0.552 in the frontal lobe. The one in the parietal lobe was 0.535, in the temporal lobe 0.690 and in the occipital lobe 0.672. Average of white matter/total area was 0.478 in the frontal lobe. The one in the parietal lobe was 0.465, in the temporal lobe 0.672 and in the occipital lobe 0.328.In alcoholics atrophy of gray matter in the frontal and occipital lobe were observed and the one of white matter were observed in the parietal and temporal lobe.