A diagnostic study on cardiomyopathy by using DNA analysis in the autopsy cases of sudden death and its application to a medicolegal judgment

点击翻译按钮获取中文摘要

MethodsIn each case which was autopsied within 24 hours after sudden cardiac death, a 10 ml blood sample was freezed at -80ﾟC and two pieces of the left cardiac ventricle and two ones of the right ventricle were fixed in 10% formaline solution.A blod sample was defrosted and DNA was extracted from isolated nuclei of peripheral white cells by procedures of sodium iodide method. A PCR amplification of exons 13 in human beta-Myosin Heavy Chain gene was done by using a set of primers. PCR-amplified fragments were broken by Dde I restriction enzyme and resolved on a 10% polyacrylamide gel run at room temperature. The gel was stained with ethidium bromide and gel bands were identified under ultraviolet light.Each piece of the cardiac ventricle was stained with hematoxylin-eosin (H-E) and azan stains and examined histopathologically.ResultsIn the first case (59-year-old male), the heart weighed 920 g. Next, in the 56-year-old male, the heart weighed 590 g. In the 63-year-old male, the one did 700 g. In the 48-year-old male, the one did 530 g. In the 27-year-old male, the one did 450 g. In the 65-year-old male, the one did 920 g. In the 25-year-old male, the one did 680 g. In the 48-year-old male, the one did 400 g.In a DNA analysis, 161 basepair fragment was identified and 129 basepair fragment was not in each case. In histopathological study on the cardiac ventricles, we found myocardial disarray.