Effect of Splenectomy on gastric carcinogenesis induced by continuous administration of MNNG to rats.

脾切除对连续给予MNNG致大鼠胃癌的影响。

• 批准号: 02670571
• 负责人: SEKIKAWA Takayoshi
• 金额: $ 0.9万
• 依托单位: Yamanashi Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670571/
• 关键词: Splenectomy; Experimental Stomach Cancer; MNNG; 実験肝癌; 脾免疫能

We examined the effect of splenectomy on the gastric carcinogenesis induced by administration of N-methyl-N'-nitro-N-nitrosoguanidine(KNNG)to rat.Three groups of 20 male Wistar-strain rats, 6 weeds old, received MNNG in the drinking water ad libitum at concentrations of 80, 100 and 120 mug/ml, respectively, for 44 weeks. In order to study the role of splenic function on the gastric carcinogenesis, we classified 20 rats to four groups, I group, sham operation before administration of MNNG, (sham G.) (n=5), II group, splenectomy before administration of MNNG (spx G.) (n=5), III group, splenectomy after 2 months from administration of MNNG (2M spx G.) (n=5) IV group, splenectomy after 4 months from administration of MNNG (4M spx G.) (n=5).The animals were carefully autopsied when after receiving MNNG continuously for 44 weeks.The numbers, sizes and histological study of the tumors in the glandular stomach were examined.In 60 rats, 31 adenocarcinomas were found. The incidences of gastric c … More ancer of each group were as follows, in 80, mug/ml MNNG concentration, sham G. 2/5(40%), spx G. 2/4(50%), 2M spx G. 3/5(60%)and 4M spx G. 3/4(75%) ; in 100, mug/ml MNNG concentration, 2/5, 1/5, 2/5, 3/5 ; in 120mug/ml MNNG concentration, 2/5, 1/4, 3/5, 3/5, respectively. So the incidence of gastric cancer was the highest in the splenectomyed group after 4 months. The mean sizes of the gastric carcinomas were 4.0<plus-minus>2. Omm in the sham G. (6), 11.4<plus-minus>14.4mm in the spx G. (5), 5.2<plus-minus>3.7mm in the 2M spx G. (9)and 4.9<plus-minus>2.4mm in the 4H spx G. (11). The depth of invation of the gastric carcinomas were 3 within submucosal layer(sm)and 3 beyond muscle layer(pm)in the sham G. ; 2 within sm and 3 beyond pm in the spx G., 5 within sm and 4 beyond pm in 2M spx G. and 10 within sm and I beyond pm in 4These results suggested that splenectomy maight have the progressive effect on the gastric carcinogenesis after 4 months in the course of continuous administraion of MNNG. Less

我们研究了脾切除术对大鼠给予N-甲基-N '-硝基-N-亚硝基胍（KNNG）诱导的胃癌发生的影响。三组20只雄性Wistar系大鼠，6周龄，分别以80，100和120 μ g/ml的浓度在饮用水中自由饮用MNNG，持续44周。为探讨脾功能在胃癌发生中的作用，将20只大鼠分为4组，I组：假手术组，假手术前给予MNNG;（n=5），Ⅱ组（n = 5），在MNNG（spx G.）Ⅲ组（n=5），MNNG（2M spx G.）（n=5）IV组，给予MNNG（4 M spx G.）在连续给予MNNG 44周后，对60只大鼠进行解剖，观察腺胃肿瘤的数目、大小及组织学改变，发现腺癌31例，胃癌发生率为100%，其中10只大鼠的腺癌发生率为100%，10只大鼠的腺癌发生率为100%，10只大鼠的腺癌发生率为100%，10只大鼠的腺癌发生率为100%。 ...更多信息 各组肿瘤细胞分别在80 μ g/ml MNNG浓度下，sham G. 2/5（40%），spx G. 2/4（50%），2 M spx G。3/5（60%）和4 M spx G. MNNG浓度为100 μ g/ml时，分别为2/5、1/5、2/5、3/5 ; MNNG浓度为120 μ g/ml时，分别为2/5、1/4、3/5、3/5。脾切除组术后4个月胃癌发生率最高。胃癌的平均大小为<plus-minus>4.02。噢，在假G。(6)、11.4 ~ <plus-minus>14.4mm。(5)2M <plus-minus>spx G.（9）和4.9 ~ <plus-minus>2.4mm。（十一）、假手术组胃癌浸润深度为粘膜下层3例（sm），肌层外3例（pm）。在spx G中，2个在sm内，3个在pm外，在2 M spx G中，5个在sm内，4个在pm外。4例中10例在sm以内，1例在pm以上。提示在MNNG连续给药4个月后，脾切除对胃癌的发生有促进作用。少