Analysis of the mechanisms of down-regulation of signal transducing CD3 zeta molecules on T cells in patients with cancer-Its correlation with surgical stress.

在癌症患者中，我们评估了手术应激诱导T细胞表面CD3 Zeta分子信号转导的变化。分别于术前、术后第2、3、7天(POD)采集PBL。用流式细胞仪结合细胞内染色技术定量检测外周T细胞表面CD3Zeta分子的水平。CD3Zeta分子的表达在术后第2、3天较术前水平下调。术后7d，手术应激诱导的CD3Zeta分子表达下降，恢复至术前水平。术后第2天单核细胞与术前PBL共培养可诱导CD3Zeta分子表达下调，提示手术应激诱导T细胞CD3Zeta分子表达下调，这种下调是由单核细胞介导的。此外，初步结果表明，POD第2天单核细胞可诱导T细胞凋亡，但这一现象尚需进一步研究证实。

In cancer patients, we evaluated the alteration of signal transducing CD3 zeta molecules on T cells induced by surgical stress. PBLs were taken preoperatively, 2nd, 3rd and 7th postoperative days(POD). The levels of CD3 zeta molecules on peripheral T cells were quantified by flow cytometry with intracellular staining. The expression of CD3 zeta molecules was down-regulated on 2nd and 3rd POD compared to preoperative levels. Then, decreased expression of CD3 zeta molecules induced by surgical stress were restored to the preoperative levels on 7th POD.Co-culture of monocyte on 2nd POD with PBLs from preoperative periods induced down-regulation of CD3 zeta, indicating that surgical stress induced down-regulation of CD3 zeta molecules on T cells, which were mediated by monocyte. Moreover, preliminary results indicated that monocyte on 2nd POD induced apoptosis of T cell, however, further investigation need to prove this phenomenon.