Analysis of the mechanisms of down-regulation of signal transducing CD3 zeta molecules on T cells in patients with cancer-Its correlation with surgical stress.

癌症患者T细胞信号转导CD3 zeta分子下调机制分析——与手术应激的相关性

• 批准号: 09671292
• 负责人: SEKIKAWA Takayoshi
• 金额: $ 1.92万
• 依托单位: FACULTY OF MEDECINE,YAMANASHI MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671292/
• 关键词: surgical stress,; T cell function

In cancer patients, we evaluated the alteration of signal transducing CD3 zeta molecules on T cells induced by surgical stress. PBLs were taken preoperatively, 2nd, 3rd and 7th postoperative days(POD). The levels of CD3 zeta molecules on peripheral T cells were quantified by flow cytometry with intracellular staining. The expression of CD3 zeta molecules was down-regulated on 2nd and 3rd POD compared to preoperative levels. Then, decreased expression of CD3 zeta molecules induced by surgical stress were restored to the preoperative levels on 7th POD.Co-culture of monocyte on 2nd POD with PBLs from preoperative periods induced down-regulation of CD3 zeta, indicating that surgical stress induced down-regulation of CD3 zeta molecules on T cells, which were mediated by monocyte. Moreover, preliminary results indicated that monocyte on 2nd POD induced apoptosis of T cell, however, further investigation need to prove this phenomenon.

在癌症患者中，我们评估了手术应激诱导的T细胞上信号转导CD3 zeta分子的改变。在术前、术后第2、3和7天（POD）采集PBL。通过细胞内染色的流式细胞术定量外周T细胞上的CD3 ζ分子的水平。术后第2、3天CD3 zeta分子表达较术前明显下调。术后第7天，手术应激诱导的T细胞表面CD3 zeta分子表达下降，恢复到术前水平。术后第2天，手术应激诱导的T细胞表面CD3 zeta分子表达下调，这是由单核细胞介导的。此外，初步结果表明，第二POD单核细胞诱导T细胞凋亡，但进一步的研究需要证明这一现象。