Continuous measurement of lactate concentration in cerebral interstitium as an index of regional brain glucose metabolism in rats.

使用微透析连续测量乳酸浓度如Circ. Shock， 1992所示，通过在线混合透析液和试剂溶液连续测量乳酸浓度。反应时间约为4分钟，不足以测量脑活动变化引起的葡萄糖代谢变化。因此，我们应用该在线系统测量大鼠急性出血后骨骼肌间质间质乳酸浓度。从血乳酸浓度和肌间质乳酸浓度的比较，提示急性出血后大鼠骨骼肌是乳酸的消耗者，而不是乳酸的产生者。这一假设通过在体标定法得到了证实。生理学杂志。1992。[2]微透析法间歇测定不同模型脑间质乳酸在缺氧缺氧和内毒素休克模型中每隔10 ~ 30min测定脑间质乳酸。这些研究表明，脑乳酸浓度保持在较低水平，而血乳酸浓度明显升高。（提交出版）。还发现gaba相关药物，包括巴比妥酸盐，在微透析灌注液中加入这些物质，局部刺激后可增加局部脑乳酸浓度。[3]微透析法测定内源性神经活性物质清醒大鼠出血诱导的局部脑促甲状腺素释放激素（TRH）释放。出血后，脑中隔的TRH浓度显著升高，但其他脑区仍保持基础水平（生命科学，1991）。

[1]Continuous lactate measurement using a microdialysisAs shown in Circ. Shock, 1992, labtate concentration was measured continuously via the on-line mixing of dialysate with a reagent solution. The response time was approximately 4 min, which was not rapid enough for measurement of changes in glucose metabolism caused by brain activity changes. Therefore we applied this on-line system to measurement of the interstitial lactate concentrations in skeletal muscle interstitium of the rat shortly after an acute hemorrhage. From the comparison of lactate concentrations in blood and in the muscle interstitium, it was suggested that rat skeletal muscle functions as a lactate consumer, rather than a lactate producer after acute hemorrhage. This hypothesis was confirmed by using in vivo calibration method in Am. J. Physiol. 1992.[2]Intermittent measurement of cerebral interstitial lactate in various models by a microdialysisCerebral interstitial lactate was measured with 10 - 30 min intervals in hypoxic hypoxia and in endotoxin shock models. These studies revealed that cerebral lactate concentration remained relatively at low levels while blood lactate increased markedly. (Submitted for publication). It is also found that GABA-related drugs including barbiturates increased local cerebral lactate concentration after local stimulation with these substances added in the microdialysis perfusate.[3]Measurement of endogenous neuroactive substances by a microdialysisHemorrhage-induced regional brain thyrotropin-releasing hormone(TRH)release was measured in conscious rats. The TRH concentration significantly increased in the septum after a hemorrhage but remained at basal levels in the other brain areas measured(Life Science 1991).