Continuous measurement of lactate concentration in cerebral interstitium as an index of regional brain glucose metabolism in rats.

连续测量脑间质乳酸浓度作为大鼠局部脑葡萄糖代谢指标。

• 批准号: 02670690
• 负责人: OKUDA Chieko
• 金额: $ 1.41万
• 依托单位: Kyoto Prefectural Univ. of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670690/
• 关键词: lactate; brain glucose metabolism; microdialysis; continuous measurement; shock; neurotransmitter; 脳糖代謝率; 脳内乳酸濃度; 乳酸連続測定; 循環ショック

[1]Continuous lactate measurement using a microdialysisAs shown in Circ. Shock, 1992, labtate concentration was measured continuously via the on-line mixing of dialysate with a reagent solution. The response time was approximately 4 min, which was not rapid enough for measurement of changes in glucose metabolism caused by brain activity changes. Therefore we applied this on-line system to measurement of the interstitial lactate concentrations in skeletal muscle interstitium of the rat shortly after an acute hemorrhage. From the comparison of lactate concentrations in blood and in the muscle interstitium, it was suggested that rat skeletal muscle functions as a lactate consumer, rather than a lactate producer after acute hemorrhage. This hypothesis was confirmed by using in vivo calibration method in Am. J. Physiol. 1992.[2]Intermittent measurement of cerebral interstitial lactate in various models by a microdialysisCerebral interstitial lactate was measured with 10 - 30 min intervals in hypoxic hypoxia and in endotoxin shock models. These studies revealed that cerebral lactate concentration remained relatively at low levels while blood lactate increased markedly. (Submitted for publication). It is also found that GABA-related drugs including barbiturates increased local cerebral lactate concentration after local stimulation with these substances added in the microdialysis perfusate.[3]Measurement of endogenous neuroactive substances by a microdialysisHemorrhage-induced regional brain thyrotropin-releasing hormone(TRH)release was measured in conscious rats. The TRH concentration significantly increased in the septum after a hemorrhage but remained at basal levels in the other brain areas measured(Life Science 1991).

[1]使用微透析连续测量乳酸如循环所示。 Shock，1992 年，通过透析液与试剂溶液的在线混合连续测量实验室酸盐浓度。响应时间约为4分钟，对于测量大脑活动变化引起的葡萄糖代谢变化来说还不够快。因此，我们应用该在线系统来测量急性出血后不久的大鼠骨骼肌间质中的间质乳酸浓度。通过比较血液和肌肉间质中的乳酸浓度，表明大鼠骨骼肌在急性出血后充当乳酸消耗者，而不是乳酸产生者。 Am 中使用体内校准方法证实了这一假设。 J.生理学。 1992.[2]通过微透析间歇测量各种模型中的脑间质乳酸在缺氧和内毒素休克模型中以10-30分钟的间隔测量脑间质乳酸。这些研究表明，脑乳酸浓度保持在相对较低的水平，而血乳酸显着升高。 （提交出版）。还发现，微透析灌流液中添加的巴比妥类等GABA相关药物，在局部刺激后，局部脑乳酸浓度升高。[3]通过微透析测定内源性神经活性物质，在清醒大鼠中测定出血引起的脑部促甲状腺素释放激素（TRH）释放。出血后，鼻中隔中的 TRH 浓度显着增加，但在其他测量的大脑区域中仍保持在基础水平（Life Science 1991）。

项目成果

Okuda,C.et al: "Hemorrhageーinduced regional brain thyrotropinーreleasing hormone release in conscious rats measured by microdialysis" Life Sciences. 47. 1997-2002 (1990)

Okuda, C. 等人：“通过微透析测量清醒大鼠出血引起的区域性脑促甲状腺素释放激素释放”，生命科学 47。1997-2002 (1990)。

Okuda,C.et al: "Lactate in rat skeletal muscle after hemorrhage measured by a microdialysis probe calibrated in situ" Am.J.Physiol.(1992)

Okuda,C.等人：“通过原位校准的微透析探针测量出血后大鼠骨骼肌中的乳酸”Am.J.Physiol.(1992)

Okuda C., Sawa T., Harada M., Murakami T. Matsuda T. and Tanaka Y.: "Lactate in rat skeletal muscle after hemorrhage measured by a microdialysis probe calibrated in situ" Am. J. Physiol.(1992)

Okuda C.、Sawa T.、Harada M.、Murakami T. Matsuda T. 和 Tanaka Y.：“通过原位校准的微透析探针测量出血后大鼠骨骼肌中的乳酸”Am。

Okuda C., Sawa T., Harada M., and Miyazaki M.: "Hemorrhage-induced regional brain thyrotropin-releasing hormone release in conscious rats measured by microdialysis" Life Sciences. 47. 1997-2002 (1990)

Okuda C.、Sawa T.、Harada M. 和 Miyazaki M.：“通过微透析测量清醒大鼠出血诱导的区域性脑促甲状腺素释放激素释放”生命科学。

Okuda C., Sawa T., Harada M., Murakami T. and Tanaka Y.: "Continuous measurement of lactate concentration in skeletal muscle and liver interstitium using a microdialysis method after acute hemorrhage in anesthetized rats" Circ. Shock. (1992)

Okuda C.、Sawa T.、Harada M.、Murakami T. 和 Tanaka Y.：“麻醉大鼠急性出血后使用微透析方法连续测量骨骼肌和肝间质中的乳酸浓度”Circ。