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Central Regulation of Autonomic Nervous System and Endocrine System during Circulatory Shock

循环性休克期间自主神经系统和内分泌系统的中枢调节

基本信息

批准号：
62570707
负责人：
OKUDA Chieko
金额：
$ 1.41万
依托单位：
Department of Anesthesiology, Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

In the early stage of circulatory shock, sympathetic reflexes and hormonal factors are known to compensate to prevent deterioration of the circulation. However, the central nervous system(CNS) mechanisms that mediate and coordinate such responses are largely unknown. This study is designed to identify the substance(s) in the brain, which are involved in central regulation of cardiovascular system during circulatory shock. I have studied the changes of brain thyrotropin-releasing hormone(TRH), as one of the candidates of such substances, released in the fourth ventricle of conscious rat after bleeding using a push-pull perfusion method. Centrally administered TRH is known to have the cardiovascular effect independent of its pituitary actions. In this study,it has been revealed that; 1) The concentration of TRH in the perfusate remained constant during the 140 min perfusion period in the control rat. 2) It was significantly increased during the first 20 min of perfusion immediately after withdrawing of 30% of the estimated total blood and it soon returned to the initial level. 3) Administration of neither normal rabbit serum nor antiserum of TRH into the ventricle had any significant effects on blood pressure or heart rate for 3 hr in the control animal. 4) After bleeding, animals that received TRH-antiserum showed a significantly greater decrease in blood pressure and longer duration of this decreased level than animals that received normal rabbit serum. The tendency of tachycardia which was seen immediately after bleeding in the latter animals was suppressed in the former animals, but no significant changes were detected. These results suggest that endogenous brain TRH is involved in the central regulation of the cardiovascular system after bleeding in conscious rats. However, it does not have any significant role in the central cardiovascular regulation in the normal state.

在循环性休克的早期阶段，交感神经反射和激素因子可以进行补偿，以防止循环恶化。然而，介导和协调此类反应的中枢神经系统（CNS）机制在很大程度上尚不清楚。本研究旨在鉴定大脑中参与循环性休克期间心血管系统中枢调节的物质。我采用推拉灌注法研究了清醒大鼠放血后第四脑室释放的脑促甲状腺素释放激素（TRH）作为此类物质的候选物之一的变化。众所周知，集中施用 TRH 具有独立于垂体作用的心血管作用。这项研究表明； 1) 在对照大鼠的140分钟灌注期间，灌注液中TRH的浓度保持恒定。 2)在抽取预计总血量的30%后，在灌注的前20分钟内显着增加，并且很快恢复到初始水平。 3)在对照动物中，将正常兔血清或TRH抗血清注入心室3小时对血压或心率均没有任何显着影响。 4) 放血后，与接受正常兔血清的动物相比，接受TRH抗血清的动物血压下降幅度明显更大，并且这种下降水平的持续时间更长。后者动物出血后立即出现的心动过速趋势在前者动物中受到抑制，但没有检测到显着变化。这些结果表明，清醒大鼠出血后，内源性脑 TRH 参与心血管系统的中枢调节。但在正常状态下，它对中枢心血管调节没有任何显着的作用。