Screening of sperm motility stimulating substances, and their application to male infertility

1本研究建立的玻璃柱-连续密度梯度离心法能从人精液中分离出运动性精子。该制剂不含精浆成分，适用于计算机辅助精子运动分析仪定量测定精子运动能力。2采用计算机辅助精子运动分析仪测定生物活性肽缓激肽、血管紧张素II和脑啡肽对高纯度人精子运动能力的影响。它们刺激精子活力，特别是在没有精浆的情况下的精子前进速度。然而，这种影响被BF精浆所消除。精浆中的蛋白酶可能使其失活。3磷酸二酯酶抑制剂Penglycifilline（PXF）在精浆存在的情况下刺激精子运动。逆行射精时，精液在膀胱内回流，甚至取代尿液进入培养液，尿液污染使精子活力降低。射精前膀胱内导入PXF的培养液对精子活力有保护作用。回收的精子没有丧失活力，提供给IVF-ET，导致1例成功妊娠。

1 The glass column-continuous density gradient centrifugation, established in the present study, was capable of separating progressively motile sperm from raw human semen. The preparation was free from seminal plasma components, and was suitable for quantitative measurement of sperm motility by using the computer assisted sperm motility analyzer.2 Effect bioligically active peptides, bradykinin, angiotensin II and enkephalin, on highly purified human sperm motility were estimated by using the computer assisted sperm motility analyzer. They stimulated sperm motility, especially for sperm-progressive velocity in the absence of seminal plasma. The effects were, however, disappeared by addition bf seminal plasma. Proteinases in seminal plasma might inactive them.3 Pentoxifilline(PXF), a phosphodiesterase inhibitor, stimulate sperm motility in the presence of seminal plasma. In the case of retro-grade ejaculation, semen was refluxed in blader, and even replaced urine to culture medium, contamination of urine decreased sperm viability. The culture medium with PXF intorduced in blader before ejaculation protected sperm viability. The recovered sperm without loss of motility was provided for IVF-ET, resulting one successful pregnancy.