Screening of sperm motility stimulating substances, and their application to male infertility

精子活力刺激物质的筛选及其在男性不育症中的应用

• 批准号: 02670754
• 负责人: KANEKO Satrou
• 金额: $ 1.41万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670754/
• 关键词: Human sperm; Sperm motility; Bradykinin; Angiotensin II; Enkepalin; Pentoxifilline; Retro-grade ejaculation; ペントキシフィリン; 男子不妊症; 運動賦活物質; 精子洗浄

1 The glass column-continuous density gradient centrifugation, established in the present study, was capable of separating progressively motile sperm from raw human semen. The preparation was free from seminal plasma components, and was suitable for quantitative measurement of sperm motility by using the computer assisted sperm motility analyzer.2 Effect bioligically active peptides, bradykinin, angiotensin II and enkephalin, on highly purified human sperm motility were estimated by using the computer assisted sperm motility analyzer. They stimulated sperm motility, especially for sperm-progressive velocity in the absence of seminal plasma. The effects were, however, disappeared by addition bf seminal plasma. Proteinases in seminal plasma might inactive them.3 Pentoxifilline(PXF), a phosphodiesterase inhibitor, stimulate sperm motility in the presence of seminal plasma. In the case of retro-grade ejaculation, semen was refluxed in blader, and even replaced urine to culture medium, contamination of urine decreased sperm viability. The culture medium with PXF intorduced in blader before ejaculation protected sperm viability. The recovered sperm without loss of motility was provided for IVF-ET, resulting one successful pregnancy.

1本研究建立的玻璃柱-连续密度梯度离心法能从人精液中分离出运动性精子。该制剂不含精浆成分，适用于计算机辅助精子运动分析仪定量测定精子运动能力。2采用计算机辅助精子运动分析仪测定生物活性肽缓激肽、血管紧张素II和脑啡肽对高纯度人精子运动能力的影响。它们刺激精子活力，特别是在没有精浆的情况下的精子前进速度。然而，这种影响被BF精浆所消除。精浆中的蛋白酶可能使其失活。3磷酸二酯酶抑制剂Penglycifilline（PXF）在精浆存在的情况下刺激精子运动。在逆行射精的情况下，精液在膀胱中回流，甚至取代尿液进入培养基，尿液的污染会降低精子的活力。射精前膀胱内导入PXF的培养液对精子活力有保护作用。回收的精子没有丧失活力，提供给IVF-ET，导致1例成功妊娠。

项目成果

T.Kobayashi,S.Kaneko,I.Hara,R.Aoki,T.Ohno,S.Nozawa: "Swim down Separation of proggressively motile sperm from poor quality human semen by the modified funnel prosedure" Archives of Andrology. 23. 17-20 (1991)

T.Kobayashi、S.Kaneko、I.Hara、R.Aoki、T.Ohno、S.Nozawa：“通过改进的漏斗程序从劣质人类精液中游下渐进运动的精子”男科学档案。

t.Kobayashi,S.Kaneko,I.Hara,I.Hara,R.Aoki,T.Ohno,S.Nozawa: "Swim down separation of progressively motile sperm from poor quality human semen by the modified funnel procedure" Archives of Andrology. 23. 17-20 (1991)

t.Kobayashi,S.Kaneko,I.Hara,I.Hara,R.Aoki,T.Ohno,S.Nozawa：“通过改进的漏斗程序从劣质人类精液中游下分离逐渐活动的精子”男科学档案

T. Kobayashi, S. Kaneko, I. Hara, R. Aoki, T. Ohno, S. Nozawa: "Swim down separation of progressively motile sperm from poor quality human semen by the modified funnel procedure." Archives of Andrology. 23. 17-20 (1991)

T. Kobayashi、S. Kaneko、I. Hara、R. Aoki、T. Ohno、S. Nozawa：“通过改进的漏斗程序，从劣质人类精液中游下分离逐渐活动的精子。”

Kaneko.S.,: "Effeet of Enkephalins on the motility of purifed human Sperm" Archieves of Andrology.

Kaneko.S.，：“脑啡肽对纯化人类精子活力的影响”男科学档案。