TRIGGER MECHANISM OF ACROSOME REACTION IN HUMAN SPERM MEDIATED BY ADENOSINE RECEPTOR

腺苷受体介导的人类精子顶体反应的触发机制

• 批准号: 10671571
• 负责人: KANEKO Satrou
• 金额: $ 1.86万
• 依托单位: TOKYO DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671571/
• 关键词: adenyl purine; adenosine; acrosome reaction; purine receptor; sperm; ATP

Removal of seminal plasma by washing induced spontaneous acrosome reaction (AR), in vitro, whereas presence of adenosine suppressed the induction. Several agonists against adenosine A1 receptor suppressed the induction of AR, and the competitive antagonists (FK-352, FK-838) against adenosine A1 receptor accellerated the induction of AR. There phenomena disappeared by the presence of L type calcium channel blocker. Agonists and antagosists against adenosine A2 receptor gave no influence for AR.Adenosine concentration in the seminal plasma was found to be more than 10 times higher than that of the serum. When compared the composition of adenyl purines in human seminal plasma and the serum, adenosine occupied more than 60% in the former, whereas more than 90% of adenyl purines were found to be ATP and ADP in the latter.Adenosine A1 receptor linked with L type calcium channel. There results suggested that the seminal plasma adenosine suppressed the induction of AR through adenosine A1 receptor. Removal of seminal plasma (wash out of adenosine) deleted the suppression, allowed to influx of calcium ion into the acrosome.

通过洗涤去除精浆诱导自发顶体反应（AR），在体外，而存在的腺苷抑制诱导。腺苷A1受体激动剂抑制AR的诱导，竞争性腺苷A1受体拮抗剂FK-352、FK-838促进AR的诱导。L型钙通道阻滞剂可使上述现象消失。腺苷A2受体激动剂和非激动剂对AR无影响。精浆腺苷浓度比血清高10倍以上。人精浆和血清中腺苷嘌呤的组成比较，前者中腺苷占60%以上，后者中腺苷嘌呤90%以上为ATP和ADP。腺苷A1受体与L型钙通道相连。提示精浆腺苷通过腺苷A1受体抑制AR的诱导。去除精浆（洗脱腺苷）消除了抑制，允许钙离子流入顶体。

项目成果

Kaneko S, et al.,: "Are there inositol 1, 4, 5-triphosphate (IP3) receptor human sperm"Life Sci,. 65. 135-143 (1999)

Kaneko S 等人：“人类精子中是否存在肌醇 1,4,5-三磷酸 (IP3) 受体”Life Sci，。

Kuroda,Y., Kaneko,S., Yoshimura,Y., Nozawa,S., Mikoshiba,K.,: "Influence of progesterone and GABAA receptor on calcium mobilization during human sperm acrosome reaction."Arch Androl.. 42. 185-191 (1999)

Kuroda,Y.、Kaneko,S.、Yoshimura,Y.、Nozawa,S.、Mikoshiba,K.，：“黄体酮和 GABAA 受体对人类精子顶体反应期间钙动员的影响。”Arch Androl.. 42. 185

Kaneko S, et al.,: "Infulence of progesterone and GABAA receptor on calcium mobilization during human sperm acrosome reaction"Arch Androl.. 42. 185-191 (1999)

Kaneko S 等人：“人精子顶体反应期间黄体酮和 GABAA 受体对钙动员的影响”Arch Androl.. 42. 185-191 (1999)

Kuroda,Y., Kaneko,S., Yoshimura,Y., Nozawa,S., Mikosimba,K.: "Are there inositol 1,4,5-triphosphate (IP3) receptor in human sperm."Life Sci.. 65. 135-143 (1999)

Kuroda,Y.、Kaneko,S.、Yoshimura,Y.、Nozawa,S.、Mikosimba,K.：“人类精子中是否存在肌醇 1,4,5-三磷酸 (IP3) 受体。”生命科学 65