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Induction of cytokines by oral bacterial stimulation and its arthropathic properties

口腔细菌刺激诱导细胞因子及其关节病特性

基本信息

批准号：
02670824
负责人：
RIKIISHI Hidemi
金额：
$ 1.34万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

The activities of peptidoglycan(PG)fragments derived from gram-positive cocci were examined to proliferate spleen mononuclear cells(SMNC)from murine various strains and to produce cytokines in vitro, and also examined to induce the inflammatory reaction in vivo. The proliferation of SMNC from C3H/HeN mice to PG in vitro were maximum on day 3, and greater than that of SMNC from Balb/c mice. It was indicated that the responsive cells of SMNC from C3H/HeN mouse to PG were mainly Ia^+ Mac^<2+> cells. mRNA transcripts of GM-CSF were detected in the lungs of C3H/HeN mouse much stronger than that of Balb/c mouse after 3 hr of injection. Balb/c mice were susceptible to acute arthritis after PG injection, whereas C3H/HeN and C57BL/6 mice failed to produce acute arthritis. The cytoplasmic membrane protein(MAP)of Streptococcus pyogenes, but not PG and others, when incubated with human peripheral blood lymphocytes, induced polyclonal activation of T cells after 5-7 days of incubation without IL-2 production. The activated T cells included CD^<4+> CD^<8-> helper T cells. We have obtained some informations on the responsibility of mononuclear cells to bacterial cell components, especially cytokine production and activated cells. These factors form a complex net work, and a complete understanding of the mechanisms involved in the arthritis to bacterial subfraction, must await detailed studies.

本实验检测了革兰氏阳性球菌肽聚糖(PG)片段体外对小鼠脾单个核细胞(SMNC)的增殖和产生细胞因子的作用，以及在体内诱导炎症反应的活性。C3H/HEN小鼠SMNC在体外向PG的增殖在第3天达到最大，高于Balb/c小鼠的SMNC。结果表明，C3H/HEN小鼠SMNC对PG的反应细胞主要为Ia~+细胞。注射后3h，C3H/HEN小鼠肺组织中GM-CSFmRNA的表达明显高于Balb/c小鼠。注射PG后，BALB/c小鼠易患急性关节炎，而C3H/HEN和C57BL/6小鼠未发生急性关节炎。化脓性链球菌的细胞质膜蛋白(MAP)与人外周血淋巴细胞孵育5-7天后，不产生IL-2，但不能诱导PG等人外周血淋巴细胞的多克隆活化。活化的T细胞包括CD^&lt；4+&gt；CD^&lt；8-&gt；辅助性T细胞。我们已经获得了一些关于单个核细胞对细菌细胞成分，特别是细胞因子产生和激活细胞的责任的信息。这些因素形成了一个复杂的网络，而对关节炎所涉及的机制到细菌亚组分的完全了解，还必须等待详细的研究。