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Role of sulfated glycoconjugates ectodermally derived hard tissue formation

硫酸化糖复合物在外胚层衍生的硬组织形成中的作用

基本信息

批准号：
02670818
负责人：
YOSHIDA Hisaho
金额：
$ 1.34万
依托单位：
Asahi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

U1trastructural distribution and histochemical properties of sulfated glycoconjugates in ameloblasts and enamel matrices of rat and newt were investigated by use of the high iron diamine thiocarbohydrazide silver proteinate (HID-TCH-SP), enzymatic digestion methods, and 35S-autoradiography. The HID-TCH-SP staining was detected on the interdigitating cell membrane of Tomes' process, inside most secretory granules, and on the surface layer of rat prism enamel matrix. The staining resisted enzymatic digestion not only by testicular hyaluronidase but also by heparitinase. In the developing newt prismless enamel, after deposition of the mantle dentin matrix to a certain thickness, the first enamel matrix, globular in shape, appeared in juxtaposition to the dental basement membrane and tended to be intermixed with the previously deposited dentin matrix. Subsequently, enamel matrix was deposited outside (ameloblasticside) of the dental basement membrane and formed a true enamel matrix. The staining was found in the mixed matrix and was identified as chondroitin sulfate; in contrast, the true enamel matrix contained no sulfated glycoconjugates. Although newt dentin and bone matrices incorporated a very high concentration of 35S, no radioactive reaction was observed in enamel matrix. On the surface layer of developing mammalian enamel where large amounts of sulfated glycoconjugates were detected, immunolabelling of enamel protein was significantly weak. The data available suggest that certain sulfated substances, probably sulfated glycoproteins but not glycosaminoglycans, are required in the process of prismatic enamel formation; hence they represent phylogenetically advanced elements.

用高铁二胺硫代碳酰肼银蛋白（HID-TCH-SP）、酶消化法和~（35）S-放射自显影法研究了大鼠和蝾螈成釉细胞和釉基质中硫酸化糖复合物的超微结构分布和组织化学性质。HID-TCH-SP染色见于Tomes突的交错突细胞膜、大部分分泌颗粒内部和大鼠棱柱釉基质的表层。该染色不仅抵抗睾丸透明质酸酶的酶消化，还抵抗肝素酶的酶消化。在发展中的蝾螈prismless釉质，沉积后的外套牙本质基质到一定的厚度，第一釉质基质，球状的形状，出现在并列的牙齿基底膜，并倾向于与先前沉积的牙本质基质混合。随后，釉质基质沉积在牙基底膜的外侧（成釉细胞侧），形成真正的釉质基质。在混合基质中发现染色，并鉴定为硫酸软骨素;相反，真正的釉质基质不含硫酸化糖缀合物。虽然蝾螈牙本质和骨基质纳入了非常高浓度的35 S，没有观察到釉质基质中的放射性反应。在哺乳动物牙釉质的表层，检测到大量的硫酸化糖复合物，釉质蛋白的免疫标记显着弱。现有的数据表明，某些硫酸化物质，可能是硫酸化糖蛋白，但不是糖胺聚糖，在棱柱状釉质形成过程中是必需的;因此，它们代表了遗传学上的高级元素。