The ultrastructure of osteoclast ruffled border and resorption lacuna

破骨细胞皱边边界及吸收陷窝的超微结构

• 批准号: 12671793
• 负责人: YOSHIDA Hisaho
• 金额: $ 1.6万
• 依托单位: Asahi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671793/
• 关键词: Osteoclast; ruffled border; cytoskeleton; ultrastructure; deep-etching; quick freezing; 急速冷凍凍結置換; 凍結乾燥; 細胞裏打ち構造; 急速凍結凍結置換; 細胞膜裏打ち構造

We studied the ruffled border of osteoclasts at ultrastructural levels by quick-frozen, deep-etched, and rotary shadowed replicas. In freeze substituted section, the ruffled border was reported finger-shaped or foliate by Akisaka et al., 1997. However, three-dimensional structure of the ruffled border are not known. The deep-etched electron microscopy revealed the same image as freeze-substituted sections, but also provided three-dimensional information. Complicated infoldings of ruffled border was distinguished in the deep-etched replica.When the fracture plane passed through the ruffled border, the reticular network as cytocskeletal of the fingers and foliate became visible. The reticular network fills the innercellular as a cytoplasmic core. These filaments appeared to do contact with the inner surface of the ruffled border membrane. The direct relation between the intramembranos particles of the ruffled border and the reticular network, contact may be integral to association of polarization and activation with the plasma membranes for bone resorption. On the other hand, baso-lateral membran does not appeared infoldings of plasma membrane.

我们通过快速冷冻、深蚀刻和旋转阴影复制品在超微结构水平上研究了破骨细胞的皱褶边缘。在冷冻替代切片中，Akisaka等报道了指状或叶状皱褶缘，1997.然而，褶边的三维结构尚不清楚。深蚀刻电子显微镜显示了与冷冻替代切片相同的图像，但也提供了三维信息。深蚀复型中出现了复杂的皱褶内折，当断裂面穿过皱褶时，可见到指状细胞骨架和叶状细胞骨架的网状结构。网状网络作为细胞质核心充满细胞内。这些细丝似乎与皱褶缘膜的内表面接触。皱褶边缘的膜内颗粒和网状网络之间的直接关系，接触可能是与骨吸收的质膜的极化和活化的关联不可或缺的。基底外侧膜未见质膜内折。

项目成果

吉田 寿穂: "凍結技法としてのレプリカ観察法"医学生物学電子顕微鏡技術研究会誌. 16. 37-39 (2001)

Toshiho Yoshida：“作为冷冻技术的复制观察方法”医学生物学电子显微镜技术研究会杂志 16. 37-39 (2001)。

吉田 寿穂: "凍結技法としてのレプリカ観察法"医学生物学電子顕微鏡技術学会誌. 16(1). 37-39 (2001)

Toshiho Yoshida：“作为冷冻技术的复制观察方法”医学生物学和电子显微镜技术学会杂志 16(1) 37-39 (2001)。

Yoshida, Hisaho: "Method of Preparation for Quick-Freezing Replica"J. Electr. Morose. Technol. Med. Biol.. 16 (1). 37-39 (2001)

吉田久保：“速冻复制品的制备方法”J。