Antiplaque Activity of Fluoride Releasing Resin

人们已经认识到，从牙科材料中有益地释放氟离子会增加牙齿结构中的氟化物含量，并降低牙釉质和牙本质的溶解度。也有报道称，氟化物释放牙科材料表面的菌斑堆积受到抑制。然而，氟化物已经成功地融入到很少的牙科修复材料中。因此，最近开发了一种氟化物释放树脂，并有望用于预防龋齿。这种树脂会持续释放氟化物，而不会导致物理性能的降低。因此，在本研究中，我们通过检测该树脂对远缘链球菌及其合成的葡萄糖转移酶的影响，研究了该树脂的抗菌斑能力。该树脂洗脱液具有抗菌活性，并能抑制葡聚糖的产生。然而，释放的氟化物的量不足以发挥抗酶和抗菌活性。此外，用高效液相色谱法对树脂单体等树脂组分进行了定量测定，结果表明，部分洗脱单体对葡萄糖基转移酶有明显的抑制作用。由该细菌原位合成的生物体和葡聚糖，与没有氟化物释放机制的对照树脂(MF-MMA共聚物)相比，最小限度地附着在除氟树脂的表面，因为该树脂具有较小的固体临界表面张力，在该临界表面张力下发生完全润湿。因此，该树脂不符合口腔链球菌对其的粘附性。因此，这些结果表明，氟化物释放树脂具有防腐活性。从临床应用的角度来看，将对这种树脂对整个人类牙齿结构的氟化进行研究。

It has been recognized that a profittable release of fluoride ion from dental materials increased the fluoride content of tooth structure and decreased solubility of enamel and dentin. It also has been reported that plaque accumulation is inhibited on the surface of fluoride releasing dental materials. However, fluoride has been successfully incorporated into few dental restorative materials. Recently a fluoride releasing resin has thus been developed and is anticipated to be used for preventing dental caries. This resin releases fluoride constantly without the concomitant reduction in physical properties. In this research we thus studied the antiplaque ability of this resin by examining the effect of this resin on Streptococcus sobrinus and its synthesized glucosyltransferase. The eluate from this resin showed antibacterial activity and inhibited the production of glucan. However, the amount of released fluoride was not enough to exert the antienzymatic and antibacterial activity. In addition, quntitative measurement of the resin component such as resin monomer by high perforamance liquid chromatography showed that some eluted monomers significantly inhibited glucosyltransferase. The organism and glucan synthesized in situ by this bacterium, minimally adhered to the surface of fluoride releasing resin because this resin had the small critical surface tension of the solid, at which complete wetting occurs, compared to the control resin not having fluoride releasing mechanism(MF-MMA copolymer). Thus this resin discorages on adhesion of oral streptococci to it.From these results it was concluded that fluoride releasing resin will prevent dental caries by showing antilpaque activity. From a point of view of clinical application, studies will be in progress in which the fluoridation of whole human tooth structure by this resin is investigated.