Algorithmic determination of whether a protein`s cysteine residue can be replaced by selenocysteine by silent/similiar pointwise mutagenesis in DNA/RNA. Intended application to phase determination in X-ray crystallography and NMR spectroscopy.

通过 DNA/RNA 中的沉默/类似逐点诱变，算法确定蛋白质的半胱氨酸残基是否可以被硒代半胱氨酸取代。

• 批准号: 5241840
• 负责人: Professor Dr. Rolf Backofen
• 金额: --
• 依托单位: Lehrstuhl für Bioinformatik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2000
• 资助国家: 德国
• 起止时间: 1999-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5241840?language=en
• 关键词: Algorithmic; determination; whether; protein; cysteine

Using dynamic programming windowing methods, a new algorithm will be designed and implemented to determine the likelihood of whether a given protein's cysteine residue can be replaced by selenocysteine by silent/similar pointwise mutagenesis in DNA/RNA. Algorithmically predicted substitutability of cysteine by selenocysteine will be tested by laboratory experiments. Intended application concerns phase resolution in X-ray crystallography by replacing a protein by its seleno-variant (causing amorphous diffraction), and the use of radioactively labeled 75Sel for NMR spectroscopy, to provide the 3-dimensional structure of a potentially wide class of proteins.

利用动态规划窗口方法，设计并实现了一种新的算法，以确定在DNA/RNA中通过沉默/类似的点突变是否可以将给定蛋白质的半胱氨酸残基替换为硒代半胱氨酸的可能性。算法预测的半胱氨酸被硒代半胱氨酸取代的可能性将通过实验室实验进行验证。预期应用涉及x射线晶体学中的相分辨率，通过用其硒变体代替蛋白质（引起无定形衍射），以及使用放射性标记的75Sel进行核磁共振波谱，以提供潜在广泛类别的蛋白质的三维结构。