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Attenuating Effect by Dietary Fibers of Orally Given Ri-Labeled Carcinogen Through Intestinal Tract

膳食纤维通过肠道口服 Ri 标记致癌物的减弱作用

基本信息

批准号：
02671040
负责人：
YAMAMOTO Ichiro
金额：
$ 1.28万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02671040/
关键词：
Dietary Fiber Dietary Seaweeds Kelp Laver RI-Laeled Carcinogen G- H DMBA DMBA-Induced Mammary Carcinoma RI標識発がん物質体内吸収軽減標的臓器到達軽減 DNA結合軽減

项目摘要

The present study was initiated to determine how the distribution of orally given[G- ^3H]・7, 12dimethylbenz(a)anthracene(DMBA)into rats would change with a diet containing kelp or laver, both rich in dietary fiber. Female SD rats, aged 5 weeks, were fed a diet with 2% powdered Laminaria religiosa, or powdered sheet of Pophyra sp., or 1.5% cellulose powder for 4 days, and control animals were fed a basic diet. Then, [G- ^3H]・DMBA, 44.4 kBq/0.1 ml of olive oil, was given intragastrically into each rat. The animals were autopsied 3 and 24 hrs after dosing. Contents of[ ^3H] in organs, tisuues and blood were determined using a scintillation counter, then, contents of DMBA were calculated. As-the results, content of DMBA in the skin(target tissue)and blood of kelp-fed rats in each time point was remarkably less than that of control animals. In the laver-fed or cellulose-fed rats, attenuation of DMBA content in the skin and blood was observed 3 hrs after doding. In addition, determination of DMBA binding DNA in the skin of kelp-fed rats was carried out and resulted remarkably low value(0.0192 pmol/mg DNA), compared-with that of controls(0-3514 pmol/mg DNA).Judging from the above-mentioned results, it was concluded that the anticarcinogenic activity of dietary seaweeds, such as kelp and laver, may partly be due to their function as a dietary ciber, attenuating the absorption of carcinogen through intestinal tract and thereby minimizing the amount of the carcinogen to reach the target tissue.

本研究旨在确定大鼠口服含丰富膳食纤维的海带或紫菜后，[G- ^3H]·7，12二甲基苯并（a）蒽（DMBA）在体内的分布变化。5周龄雌性SD大鼠喂食含有2%海带粉或水姬草粉的饲料，或1.5%纤维素粉喂养4天，对照组动物饲喂基础饲料。然后，每只大鼠灌胃给予44.4 kBq/0.1 ml橄榄油的[G- ^3H]·DMBA。给药后3和24小时对动物进行尸检。用闪烁计数器测定各器官、组织和血液中[ ^3H]的含量，计算DMBA的含量。结果表明，各时间点喂海带大鼠皮肤（靶组织）和血液中DMBA含量均显著低于对照组。在喂饲紫菜或纤维素的大鼠中，在给药后3小时观察到皮肤和血液中DMBA含量的减少。此外，还测定了喂海带的大鼠皮肤中DMBA结合DNA的值，（0.0192 pmol/mg DNA），与对照组相比（0-3514 pmol/mg DNA）.从上述结果可以得出结论，食用海藻如海带和紫菜的抗癌活性可能部分是由于它们作为膳食纤维的功能，减弱致癌物通过肠道的吸收，从而使到达靶组织的致癌物的量最小化。