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Growth hormone-releasing factor and neuroactive substances (galanin, neurotensin, dopamine)-containing neurons projecting to the median eminence of the rat.

含有生长激素释放因子和神经活性物质（甘丙肽、神经降压素、多巴胺）的神经元投射到大鼠的正中隆起。

基本信息

批准号：
02671101
负责人：
NIIMI Michio
金额：
$ 0.13万
依托单位：
Kagawa Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

1. Galanin(GAL)and growth hormone-releasing factor(GRF)-containing neurons projecting to the median eminence of the rat ------- Through the combined demonstration of the retrograde transport of True blue and the immunohistochemical staining of GAL, the GAL neurons that project to the median eminence were identified. Moreover, the distribution of GAL and growth hormone-releasing factor(GRF)was analyzed in the arcuate nucleus(ARC)with an elution-restaining procedure. Following the injection of True blue into the median eminence, GAL-positive cells labeled with True blue were found mainly in the ARC. The elution-restaining results revealed that many retrogradely labeled GAL neurons in the ARC contained GRF. These findings suggest that a subpopulation of GAL neurons in the ARC sending axons to the median eminence produces, stores and releases a GRF-like peptide.2. Neurotensin(NT)and GRF-containing neurons projecting to the median eminence of the rat The retrogradely labeled NT neurons were found mainly in the ARC. Only a few'labeled NT neurons were seen in the periventricular and the paraventricular nuclei. The elution restaining procedur demonstrated that an average of 43% of the labeled NT cells in the ventral part of the ARC restained for GRF.3. Dopamine(DA)and GRF-containing neurons projecting to the median eminence of the rat. Approximately 56% of the tyrosine hydroxylase(TH)neurons in the ARC were labeled with True blue, indicating that they projected to the median eminence. The elution restaining procedure revealed that an average of 32% of the labeled TH cells in the ventrolateral portion of the ARC contained GRF. These findings support the hypothesis of co-expression of dopamine with GRF from the ARC.

1.投射到大鼠正中隆起的甘丙素(GAL)和生长激素释放因子(GRF)神经元-通过True Blue逆行运输和GAL免疫组织化学染色相结合的方法，鉴定了投射到正中隆起的GAL神经元。用放散抑制法分析GAL和生长激素释放因子(GRF)在弓状核(ARC)中的分布。在正中隆起注射True Blue后，True Blue标记的GAL阳性细胞主要分布在ARC。放散抑制结果显示，ARC内许多逆行标记的GAL神经元含有GRF。这些发现表明，ARC中向正中隆起发送轴突的GAL神经元亚群产生、储存和释放一种GRF样肽。投射到大鼠正中隆起的神经降压素(NT)和GRF神经元，逆行标记的NT神经元主要见于ARC。在脑室周围核和室旁核中仅见少量NT标记神经元.放散抑制实验表明，ARC腹侧部标记的NT细胞中，平均有43%的细胞对GRF.3有抑制作用。多巴胺(DA)和GRF能神经元投射到大鼠的正中隆起。ARC内约56%的酪氨酸羟化酶(TH)神经元被True Blue标记，表明它们投射到正中隆起。放散抑制实验显示，ARC腹外侧部标记的TH细胞中平均有32%含有GRF。这些发现支持多巴胺与来自ARC的GRF共同表达的假说。