Multiple Sessions of Regenerative Electrical Stimulation to Accelerate Sensory Neuron Regeneration

项目摘要 周围神经损伤(PNIS)影响年富力强的男性和女性， 运动和感觉功能以及神经病理性疼痛。再生电刺激已被证明 以加速PNI后的功能恢复。临床前研究表明，多个一小时 再生促进电刺激可以进一步加速运动功能的恢复， 但对感官再生有不利影响。确定一组参数的调查 这对运动和感觉都有好处，所以需要多次再生刺激 成为一种可能的治疗方法。为了评估不同时长的刺激会话，一些再生 相关基因将被评估：甘丙氨酸(GAL)、LIF和GAP 43，但以前的研究没有 电刺激治疗后评估GAL或LIF。我假设，减少的长度 刺激将允许增加刺激会话的次数，同时仍允许 LIF和GAL表达增强。为了评估这一点，我将确定LIF和GAL是否出现故障- 单侧坐骨神经再生促进电刺激3小时后的调节 如果是这样，将使用LIF和具有GAP 43的GAL来确定多个 促进感觉神经再生的刺激环节。该表达式将通过 LIF、GAL和GAP 43的RNAScope，这是我将从齐格蒙德实验室学到的技术。这个 然后将使用找到的参数来确定它们是否会加速再生。再生将是 通过冯·弗雷细丝进行评估，这是我将从齐格蒙德实验室学习的另一项技术。

Project Summary Peripheral nerve injuries (PNIs) affect men and women in the prime of their lives, with the loss of motor and sensory function and neuropathic pain. Regenerative electrical stimulation has been shown to accelerate functional recovery after a PNI. Preclinical studies have shown that multiple one-hour sessions of regenerative promoting electrical stimulation can further accelerate motor function recovery, but have a detrimental effect on sensory regeneration. An investigation to determine a set of parameters that would benefit both motor and sensory is needed so multiple regenerative stimulation sessions can become a possible treatment. To evaluate different lengths of stimulation sessions, a few regenerative associated genes will be evaluated: galanin (GAL), LIF, and GAP 43, but previous studies have not evaluated GAL or LIF after electrical stimulation treatment. I hypothesize that decreasing the length of the stimulation will allow for increasing the number of stimulation sessions while still allowing enhanced LIF and GAL expression. To assess this, I will determine if LIF and GAL are down- regulated after three hours of regenerative promoting electrical stimulation after a unilateral sciatic nerve injury; if so, LIF and GAL with GAP 43 will be used to determine a set of parameters for multiple stimulation session that promote sensory nerve regeneration. The expression will be determined via the RNAscope of LIF, GAL, and GAP 43, a technique I will learn from the Zigmond laboratory. The parameters found will then be used to determine if they accelerate regeneration. Regeneration will be assessed via von Fray filaments, another technique I will learn from the Zigmond lab.