Molecular principles of cell movements in Dictyostelium discoideum

盘基网柄菌细胞运动的分子原理

• 批准号: 5242408
• 负责人: Professor Dr. Michael Schleicher
• 金额: --
• 依托单位: Institut für Zellbiologie (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2000
• 资助国家: 德国
• 起止时间: 1999-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5242408?language=en
• 关键词: Molecular; principles; cell; movements; Dictyostelium

Directed cell motion during chemotaxis along a chemical gradient is a ubiquitous phenomenon, found in many organisms and specialized cells during e.g. development, cell aggregation, inflammation, angiogenesis, wound healing etc.. The set of molecular components necessary for coordinated directed cell movement is certainly very large and might be as complex as the crosstalk between the numerous proteins at focal adhesions. At present, only a small but steadily increasing number of proteins is known to be involved in cellular motility at the moving front of Dictyostelium amoebae, currently the best model system for molecular studies on directed cell movement. The available data point out that an extensive crosstalk between proteins below the plasma membrane regulates directed cell motility and that especially distinct binding domains like pleckstrin-homology, poly-proline, actin-binding, PIP2-binding, or WD domains are directly involved in the targeted localization of soluble factors. The proteins with impact on directed cell movement will be identified by scanning data from the current Dictyostelium genome projects, fusing the genes of interest to GFP and analyzing the subcellular redistribution of tagged gene products during chemotactic motility.

在趋化过程中，细胞沿着化学梯度定向运动是一种普遍存在的现象，在许多生物体和特化细胞中都有发现，例如发育、细胞聚集、炎症、血管生成、伤口愈合等过程。协调的定向细胞运动所必需的分子组分当然是非常大的，可能像许多蛋白在局灶黏附时的串扰一样复杂。目前，已知只有少量但数量稳步增加的蛋白质参与变形虫Dictyostelium amoebae的运动前部的细胞运动，变形虫Dictyostelium amoebae是目前分子研究定向细胞运动的最佳模型系统。现有数据表明，质膜下蛋白质之间的广泛串扰调节着定向细胞运动，特别是不同的结合域，如pleckstrin-homology， poly-脯氨酸，肌动蛋白结合，pip2结合或WD结构域直接参与可溶性因子的靶向定位。对定向细胞运动有影响的蛋白质将通过扫描当前盘基骨柱基因组计划的数据来鉴定，融合GFP感兴趣的基因，并分析在趋化运动过程中标记基因产物的亚细胞再分配。