The role of fatty acid binding proteins in the binding and transport of lipophilic drugs

脂肪酸结合蛋白在亲脂性药物的结合和转运中的作用

• 批准号: DP0342458
• 负责人: Prof Martin Scanlon
• 金额: $ 17.8万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0342458
• 关键词: role; fatty; acid; binding; proteins

Poorly water-soluble drugs must cross the aqueous cytoplasm of intestinal cells if they are to be absorbed following oral administration. The mechanisms by which this occurs are currently unknown. We have shown that some lipophilic drugs are capable of binding to cytosolic transport proteins called fatty acid binding proteins (FABPs). We propose to use a range of physical techniques including NMR spectroscopy, calorimetry and fluorescence firstly to identify the nature of drug binding to FABP and secondly to determine the effect of binding on drug transport.

水溶性差的药物如果要在口服给药后被吸收，则必须穿过肠细胞的含水细胞质。这种情况发生的机制目前尚不清楚。我们已经表明，一些亲脂性药物能够结合到胞质转运蛋白称为脂肪酸结合蛋白（FABP）。我们建议使用一系列的物理技术，包括NMR光谱，量热法和荧光首先确定药物结合FABP的性质，其次确定药物转运的结合效果。