Multipatch Setup

我们特此申请采购多贴片装置，使美因茨大学医学中心的神经科学研究小组能够进行复杂的电生理实验。多贴片设置可以直接分析网络参数，如连通性，兴奋和抑制，个体动作电位的时间精确检测，以及急性脑切片中突触特性（短期可塑性，突触强度等）的分析。原则上，通过记录2个神经元的传统设置，也可以分析突触通信。然而，这些测量非常耗时，因为在急性脑切片中发现单突触连接对的概率非常低。利用所提出的多贴片设置，最多可以同时记录12个神经元。此外，每次记录后，装置上的移液器可以自动清洗，这样就可以用一个移液器连续记录来自多个神经元的信号。这大大增加了可以在一个实验中测试的神经元连接的数量（bbb100）。在多贴片装置的帮助下，可以在短时间内获得大量的电生理数据，大大减少了测试动物的数量。这将使我们能够对急性脑切片中复杂的网络参数进行全面分析，这是传统装置不可能实现的，因为它需要大量的时间。

We hereby apply for the procurement of a multipatch setup that will enable neuroscience researching groups at the University Medical Center Mainz to carry out complex electro-physiological experiments. A multipatch setup enables a direct analysis of network parameters such as connectivity, excitation and inhibition, time-precise detection of individual action potentials, and the analysis of synapse properties (short-term plasticity, synaptic strength, etc.) in acute brain slices. In principle, the analysis of synaptic communication is also possible on conventional setups with recording form 2 neurons. However, these measurements are very time-consuming, since the probability of finding monosynaptically connected pairs in acute brain slices is extremely low. With the proposed multipatch setup, up to 12 neurons can be recorded simultaneously. In addition, the pipettes on the setup can be cleaned automatically after each recording such that one can record with one pipette signals from several neurons in succession. This drastically increases the number of neuron connections that can be tested in one experiment (>100). With the help of a multipatch setup, large amounts of electrophysiological data can be obtained in a short time with a significantly reduced number of test animals. This will enable us to carry out a comprehensive analysis of complex network parameters in acute brain slices, which would not be possible with conventional setups the high expenditure of time.