LC/MS2 Setup for Standard, as well as High Sensitivity Proteomics Work-Flows

使用基于质谱学的方法对蛋白质混合物进行全球分析已经发展成为生物医学研究的基石。在马尔堡菲利普斯大学医学系，这种分析被广泛使用，并与化学系的质谱学和元素分析服务组合作进行。目前的样品光谱范围从亲和纯化蛋白复合体、全细胞和组织裂解物到翻译后修饰(PTM)的全球分析。在医学部新成立的翻译蛋白质组学研究所和核心设施(自10.2021以来)的背景下，目标是建立包括高效液相色谱在内的高分辨率质谱仪。这些仪器倾向于a)扩大现有仪器的能力，目前这一能力不足以满足日益增长的高需求；b)扩展能力，以处理极低可获得性的分析物，特别是单细胞蛋白质组学方法。

The global analysis of protein mixtures using mass spectrometry-based methods hasevolved into a corner stone of biomedical research. At the Department of Medicine ofthe Philipps-Universität of Marburg, such analyses are intensively used and performedin cooperation with the Service Group Mass Spectrometry and Elemental Analysis atthe Department of Chemistry. The current sample spectrum ranges in complexity andcorrelated analytical demand from affinity purified protein complexes, via whole cell andtissue lysates to global analyses of post-translational modifications (PTMs).In the context of the new establishment of an Institute and Core Facility of TranslationalProteomics at the Department of Medicine (since 10.2021), a high resolution massspectrometer setup is targeted including liquid chromatography. The instruments are in-tended to a) expand existing instrument capacity, which is currently insufficient to meethigh and growing demand and b) extend capabilities towards tackling analytes of ex-tremely low availability, in particular single cell proteomics approaches.