LC/MS2 Setup for Standard, as well as High Sensitivity Proteomics Work-Flows

使用基于质谱的方法对蛋白质混合物进行全球分析已经发展成为生物医学研究的基石。在马尔堡的菲利普斯大学医学系，这种分析被广泛使用，并与化学系的质谱和元素分析服务组合作进行。目前的样品光谱范围在复杂性和相关的分析需求从亲和纯化的蛋白质复合物，通过全细胞和组织裂解物的翻译后修饰（PTM）的全球分析。在新成立的翻译蛋白质组学研究所和核心设施的背景下，在医学系（自2021年10月），高分辨率质谱仪设置的目标包括液相色谱。这些仪器旨在a）扩展现有仪器容量，目前该容量不足以满足高且不断增长的需求，以及B）扩展处理可用性极低的分析物的能力，特别是单细胞蛋白质组学方法。

The global analysis of protein mixtures using mass spectrometry-based methods hasevolved into a corner stone of biomedical research. At the Department of Medicine ofthe Philipps-Universität of Marburg, such analyses are intensively used and performedin cooperation with the Service Group Mass Spectrometry and Elemental Analysis atthe Department of Chemistry. The current sample spectrum ranges in complexity andcorrelated analytical demand from affinity purified protein complexes, via whole cell andtissue lysates to global analyses of post-translational modifications (PTMs).In the context of the new establishment of an Institute and Core Facility of TranslationalProteomics at the Department of Medicine (since 10.2021), a high resolution massspectrometer setup is targeted including liquid chromatography. The instruments are in-tended to a) expand existing instrument capacity, which is currently insufficient to meethigh and growing demand and b) extend capabilities towards tackling analytes of ex-tremely low availability, in particular single cell proteomics approaches.