LC/MS2 Setup for Standard, as well as High Sensitivity Proteomics Work-Flows

适用于标准和高灵敏度蛋白质组学工作流程的 LC/MS2 设置

• 批准号: 533391421
• 金额: --
• 依托单位: Philipps-Universität Marburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/533391421?language=en
• 关键词: LC; MS2; Setup; Standard; well

The global analysis of protein mixtures using mass spectrometry-based methods hasevolved into a corner stone of biomedical research. At the Department of Medicine ofthe Philipps-Universität of Marburg, such analyses are intensively used and performedin cooperation with the Service Group Mass Spectrometry and Elemental Analysis atthe Department of Chemistry. The current sample spectrum ranges in complexity andcorrelated analytical demand from affinity purified protein complexes, via whole cell andtissue lysates to global analyses of post-translational modifications (PTMs).In the context of the new establishment of an Institute and Core Facility of TranslationalProteomics at the Department of Medicine (since 10.2021), a high resolution massspectrometer setup is targeted including liquid chromatography. The instruments are in-tended to a) expand existing instrument capacity, which is currently insufficient to meethigh and growing demand and b) extend capabilities towards tackling analytes of ex-tremely low availability, in particular single cell proteomics approaches.

使用基于质谱的方法对蛋白质混合物进行全球分析已经发展成为生物医学研究的基石。在马尔堡Philipps-Universität医学院，这种分析被广泛使用，并与化学系的质谱分析和元素分析服务组合作进行。目前样品光谱的复杂性和相关分析需求范围从亲和纯化蛋白复合物，通过全细胞和组织裂解物到翻译后修饰（PTMs）的全局分析。在医学院新成立的翻译蛋白质组学研究所和核心设施（自2021年10月起）的背景下，高分辨率质谱仪装置包括液相色谱。这些仪器的目的是a)扩大现有仪器的能力，目前不足以满足日益增长的需求；b)扩展处理极低可用性分析物的能力，特别是单细胞蛋白质组学方法。