The structure and function of the enzymes concerned with glycolysis

在该补助金的财政支持下，于1991-1992年间对与糖酵解相关的酶进行了晶体结构分析。测定了Hata博士的木聚糖酶和纤维素酶、Matsuura博士的麦芽四糖（G4）形成外切淀粉酶、Harada博士的细菌溶菌酶的分子结构。根据目的酶的结构特点，阐明了目的酶的功能。Yamane博士、Fukuyama博士和Hamada博士等分别对细菌α-淀粉酶、α-淀粉酶抑制剂和麦芽五糖（G5）形成淀粉酶进行了先进的晶体学研究。Harada博士和Highchi博士等也改进了蛋白质的结晶技术。对于结构分析，Yamane博士、Tanaka博士等在分子置换法的方法学方面取得了重大进展，并在集体研究报告中公布了专有技术。Satow教授、Tanaka博士和Morimoto博士对硬件和软件进行了改进，以更快地收集和处理X射线数据。为了有效利用生物质能源，饭岛教授在原核细胞中克隆了β-半乳糖苷酶和纤维二糖水解酶。

The crystal structure analyses of the enzymes concerned with glycolysis have been carried out during 1991-1992 with the financial support of this Grant-in-Aid. The molecular structures of xylanase and cellulase by Dr. Hata, malto-teraose(G4) forming exo-amylase by Dr. Matsuura, bacterial lysozyme by Dr. Harada, were determined. The functions of objective enzymes were made clear on the basis of their structures. The advanced crystallographic studies were promoted on bacterial alpha-amylases by Dr. Yamane, alpha-amylase inhibitor by Dr. Fukuyama, and malto-pentaose (G5) forming amylase by Dr. Hamada, etc. The crystallization techniques of proteins were also improved, for example, by Drs. Harada and Highchi. For the structure analysis, the methodology on the molecular replacement method was made a great advance by Drs. Yamane, Tanaka, etc. and the know-how was published in the collective research reports. The improvement of the hardwares and softwares to collect and process the X-ray data more rapidly was carried out by Prof. Satow and Drs. Tanaka and Morimoto. The cloning of beta-galactosidase and cellobiohydrolase in prokaryotic cells could be executed by Prof. Iijima, in order to use biomass energy resouces effectively.