Survey on scrapie in Japanese : detection of PrP^<Sc> and studies on the distribution of the PrP genotypes in sheep.

日本痒病调查：PrP^<Sc>的检测及绵羊PrP基因型分布的研究。

• 批准号: 03304025
• 负责人: SHINAGAWA Morikazu
• 金额: $ 8.7万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03304025/
• 关键词: SCRAPIE; SURVEY; PrP^<Sc>; RFLP TYPES; スクレイピー; 海綿状脳症; PrP遺伝子型; 伝達性海綿状脳症; サ-ベイランス

1. Restriction fragment length polymorphism (RFLP) on the sheep-PrP gene : To investigate the RFLP types on the PrP-gene, chromosomal DNAs from sheep tissues collected from various districts in Japan were analyzed using restriction endonucleases EcoRI and HindIII and the coding region of sheep PrP as a probe. Sheep were classified into 6 types. Types I to III corresponded to the types reported in England. Nearly half of the scrapie sheep belonged to type I and the frequency of type II in scrapie sheep was low. Distribution of the types in different parts of Japan showed some variations.2. Survey of scrapie infected sheep in Japan : To survey the incidence of scrapie infection in Japan, detection of PrP^<Sc> in a total of 197 sheep brains, spleens and lymph nodes obtained from various districts in Japan was carried out. PrP^<Sc> was detected in 16 sheep collected in Hokkaido and in 2 obtained from other districts. However, these two sheep had also been produced in Hokkaido. These findings suggest that contamination with scrapie in Japan is restricted to sheep produced in Hokkaido.

1.绵羊PrP基因的限制性片段长度多态性(RFLP)：为研究绵羊PrP基因的RFLP类型，以EcoRI和HindIII限制性内切酶及绵羊PrP编码区为探针，对日本不同地区绵羊组织的染色体DNA进行了分析。将绵羊分为6个类型。类型I至类型III与英国报告的类型相对应。近一半的瘙痒病绵羊属于I型，而II型的出现频率很低。各类型在日本不同地区的分布表现出一定的差异。日本羊瘙痒病调查：为了解日本羊瘙痒病的发病情况，对来自日本各地的197只绵羊的脑、脾和淋巴结进行了PrP^&lt；Sc&gt；从北海道采集的16只绵羊和从其他地区采集的2只绵羊中检出PRP^&lt；SC&gt；然而，这两只羊也是在北海道饲养的。这些发现表明，在日本，羊痒病的污染仅限于北海道生产的绵羊。

项目成果

Muramatsu,y.et al.: "Detection of PrP^<Sc> in sheep at the preclinical stage of scrapie and its significance for diagnosis of insidious infection." Arch.Virol.(in press). (1994)

Muramatsu,y.et al.：“羊痒病临床前阶段 PrP^<Sc> 的检测及其对隐匿性感染诊断的意义。”

Muramatsu,Y.,et al.: "A specific RFLP type associated with the occurence of sheep scrapie in Japan." Archives of Virology. 127. 1-9 (1992)

Muramatsu,Y.,et al.：“一种与日本绵羊痒病发生相关的特定 RFLP 类型。”

Muramatsu, Y., et al.: "A specific RFLP type associated with the occurrence of sheep scrapie in Japan" Arch.Virol.127. 1-9 (1992)

Muramatsu, Y. 等人：“与日本羊痒病发生相关的特定 RFLP 类型”Arch.Virol.127。

Y.Muramatsu: "A specific RFLP type associated with the occurrence of sheep scrapie in Japan." Arch.Virol.

Y.Muramatsu：“一种与日本绵羊痒病发生相关的特定 RFLP 类型。”

Onodera,A.et al.: "Survey of natural scrapie in Japan:analysis of RFLP types of the PrP gene and detection of PrP^<Sc> mainly in Suffolk sheep." J.Vet.Med.Sci.56(in press). (1994)

Onodera,A.et al.：“日本天然痒病的调查：PrP 基因的 RFLP 类型分析以及主要在萨福克羊中检测 PrP^<Sc>”。