Studies on spore dimorphism of the microsporidia
微孢子虫孢子二态性的研究
基本信息
- 批准号:03660057
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1991
- 资助国家:日本
- 起止时间:1991 至 1993
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The purpose of present research was to make sure the development of two types of spores of Nosema bombycis in cells of the silkworm larvae, and examine other microsporidia whether they produce the two types of spores or not. Those spores were discovered first in cultured cells after N . bombycis was inoculated. One type of those spors is characterized by 3 to 5 coils of the polar tube, early development (36 h postinoculation), and the spontaneous discharge of a short polar tube. The other is characterized by 10 to 12 coils of the polar tube and the late development (54 h postinoculation).The results are summerized as below, 1) The two types of spores were confirmed to develop in midgut epithelial cells of the silkworm larvae after peroral inoculation. 2) A new Nosema sp. was isolated from the lawn grass cutworm, Spodoptera deprauata. This microsporidium produced two types of spores exactly the same ones as found in N.bombycis, while it did not infect the silkworm by oral administration of spores, and its spores did not react with latex particles whic had been sensitized with a monoclonal antibody against spores of N.bombycis. This microsporidium had two nuclei in diplokaryotic arrangement throughout its development. A meront gave rise to two spores, and there was no sporophorous vesicle. Hence it is another Nosema sp. isolated from S.deprauata. 3) We also identified the two types of spores in N.furnacalis developing in cultured cells. Furthermore, one of spore, early developing one, was isolated and successfully inoculated onto cultured cells, giving high rate of infection.These results indicate that this spore dimorphism is a character shared by microsporidia belonging to the genus Nosema.
本研究的目的是确定家蚕微孢子虫两种孢子在家蚕幼虫细胞内的发育情况,并考察其他微孢子虫是否产生这两种孢子。这些孢子是在N。接种家蚕。其中一种孢子的特征是3至5圈极管,早期发育(接种后36小时),并自发放电短极管。另一株为10 ~ 12圈极管,发育较晚(接种后54 h)。结果表明:1)经口接种后,两种孢子均可在家蚕幼虫中肠上皮细胞中发育。2)从草地草蛾(Spodoptera deprauata)中分离到一个新的小虫属。该微孢子虫产生与家蚕完全相同的两种类型的孢子,但它不通过口服孢子感染家蚕,其孢子不与用针对家蚕孢子的单克隆抗体致敏的乳胶颗粒发生反应。该微孢子虫在整个发育过程中具有双核排列。单孢子生两个孢子,无孢子囊。因此,它是从s.d prauata分离出来的另一种小孢子虫。3)在培养的细胞中还发现了两种孢子类型。此外,分离出一个发育早期的孢子,成功地接种到培养细胞上,感染率高。这些结果表明,这种孢子二态性是小孢子虫属小孢子虫所共有的特征。
项目成果
期刊论文数量(21)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hidetoshi IWANO.Nobuaki SHIMIZU.Yuji KAWAKAMI and Ren ISHIHARA: "Spore dimorphism and some other biological features of a Nosema sp.isolated from the lawn grass cutworm.Spodoptera depravata Butler" Applied Entomology and Zoology (in press). 29. ??? (1994)
Hidetoshi IWANO.Nobuaki SHIMIZU.Yuji KAWAKAMI 和 Ren ISHIHARA:“从草坪草地老虎中分离出的微孢子虫的孢子二态性和一些其他生物学特征。Spodoptera depravata Butler”应用昆虫学和动物学(正在出版)。
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Hidetoshi IWANO,Nobuaki SHIMIZU,Yuji KAWAKAMI and Ren ISHIHARA: "Spore dimorphism and some other biological features of a Nosema sp.isolated from the lawn grass cutworm.Spodoptera depravata Butler" Applied Entomology and Zoology. 29(in press). (1994)
Hidetoshi IWANO、Nobuaki SHIMIZU、Yuji KAWAKAMI 和 Ren ISHIHARA:“从草坪草地老虎中分离出的微孢子虫的孢子二态性和其他一些生物学特征。Spodoptera depravata Butler”应用昆虫学和动物学。
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石原廉,岩野秀俊: "家蚕微粒子病病原の保有宿主としてのスジキリヨトウ." 日本蚕糸学雑誌. 60. 236-237 (1991)
Ren Ishihara,Hidetoshi Iwano:“条纹粘虫是蚕致病性颗粒病的宿主。”《日本丝绸科学杂志》60. 236-237 (1991)
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Iwano, H.and Ishihara, R.: "Isolation of Nosema bombycis from moths of the lawn grass cutworm, Spodoptera depravata Butler. (in Japanese)" J.Sericult.Sci.Jpn. 60. 279-287 (1991)
Iwano, H. 和 Ishihara, R.:“从草坪草地老虎蛾中分离家蚕微孢子虫,Spodoptera depravata Butler。(日语)”J.Sericult.Sci.Jpn。
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岩野秀俊,石原廉: "Nosema bombycisのスジキリヨトウ成虫からの分離." 日本蚕糸学雑誌. 60. 279-287 (1991)
Hidetoshi Iwano,Ren Ishihara:“从麻雀虫成虫中分离家蚕微孢子虫。”日本血清学杂志 60. 279-287 (1991)
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ISHIHARA Ren其他文献
ISHIHARA Ren的其他文献
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{{ truncateString('ISHIHARA Ren', 18)}}的其他基金
Detection by PCR of the silkworm infected with Nosema bombycis
家蚕感染家蚕微孢子虫的PCR检测
- 批准号:
06660068 - 财政年份:1994
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)