Novel peptidylglycans with hemagglufinating activity-chemical structure and biological activity

具有血凝活性的新型肽聚糖-化学结构和生物活性

基本信息

批准号：
03660211
负责人：
HORI Kanji
金额：
$ 1.41万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

We found that eleven species of marine algae possessed novel hemagglutinins, which could not be extracted with phosphate buffered saline, unless the algae had been pretreated with Pronase. The novel hemagglutinins have been isolated from the Pronase-treated extracts of the red algae, Gelidium amansii and Carpopeltis flabellata, respectively. The 'Pronase treatment-dependent' hemagglutinins were commonly peptidylglycans with the apparent molecular weight of 8 x 10^5, respectively. As to glycan moieties, the major monosaccharides were Gal and Xyl, and uronic acids, 3,6-anhydro-galactose and sulfate esters also detected as the constituents. The peptide moieties share similar amino acid composition rich in Ser, Gly and Glx, suggesting that Xyl-Ser linkages in their molecules, respectively.The 'Pronase treatment-dependent' hemagglutinins markedly agglutinated sheep erythrocytes, especially trypsin- or Pronase treated ones, and also showed mitogenic activity for splenic lymphocytes of mice. They were stable at a high temperature and in a wide range of pH with rather increase at a low pH. The activity was weakly inhibited by asialofetuin and NaCl,but not by other sugar compounds tested. The activity was not affected by periodic acid oxidation or under the dissociation state with 4M guanidine-HCl as well as digestion with enzymes such as protease, glycosidases and sulfatase, which suggest that they have chemical structures resistant to chemical or enzymatic attacks examined.

我们发现，除非藻类已用prinase预处理藻类，否则11种海洋藻类具有新型的血凝集素，无法用磷酸盐缓冲盐水提取。新型的血凝素已从红藻，明胶amansii和parpopeltis flabellata的原始酶处理提取物中分离出来。 “固酶治疗依赖性”血凝素通常是肽基甘氨酸，表观分子量分别为8 x 10^5。至于聚糖部分，主要的单糖为gal和xyl，以及尿酸，3,6-羟基乳糖糖和硫酸盐酯，也被发现为成分。肽部分具有相似的氨基酸组成，富含Ser，Gly和Glx，表明其分子中的Xyl-ser链接分别是Xyl-ser链接。“ pronase依赖性治疗依赖性”血凝素明显凝集的绵羊红细胞蛋白链接，尤其是胰蛋白酶 - 或原始酶治疗的蛋白酶或骨膜治疗的蛋白酶，也表现出了斑点味的斑点。它们在高温下稳定，在广泛的pH范围内，在低pH值下相当增加。 Asialofetuin和NaCl薄弱地抑制了该活性，但没有测试的其他糖化合物。该活性不受周期性酸氧化的影响，也不受4M鸟嘌呤-HCL的解离状态以及与蛋白酶，糖苷酶和硫酸酶等酶消化的影响，这表明它们具有对所检查的化学结构抗化学结构的化学结构。