Library constructionand biological activities of high mannose N-glycan-specific algal lectins

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We succeeded in the cDNA cloning and preparations of active recombinants of high mannose N-glycan-specific algal lectins thatcan be grouped into three types (Type I～III) based on the recognition moiety within the branched oligomannosides of the N-glycans. Furthermore,we newly isolated and characterized the lectins, which belong to type I, from the three cultivated species of edible seaweeds. We found that the type I～III lectins had the anti-HIV activity and inhibitory activity against the growth of the cultured cells which are derived from human colonic cancer. These lectins were also useful to separate easily the high mannose N-glyans to each other from their mixture.