Library constructionand biological activities of high mannose N-glycan-specific algal lectins

高甘露糖N-聚糖特异性藻凝集素的文库构建及生物活性

• 批准号: 19380122
• 负责人: HORI Kanji
• 金额: $ 12.23万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380122/
• 关键词: レクチン; 藻類; 高マンノース型糖鎖; 生物活性; 海藻; 遺伝子; 糖鎖結合特異性; 分子構造; 藍藻; 遺伝子クローニング; 組換え体; 糖鎖認識; 抗HIV

We succeeded in the cDNA cloning and preparations of active recombinants of high mannose N-glycan-specific algal lectins thatcan be grouped into three types (Type I～III) based on the recognition moiety within the branched oligomannosides of the N-glycans. Furthermore,we newly isolated and characterized the lectins, which belong to type I, from the three cultivated species of edible seaweeds. We found that the type I～III lectins had the anti-HIV activity and inhibitory activity against the growth of the cultured cells which are derived from human colonic cancer. These lectins were also useful to separate easily the high mannose N-glyans to each other from their mixture.

我们成功地基于基于n-聚糖的分支寡素糖苷内的识别部分，可以将高甘露糖N-聚糖特异性藻类特异性藻类讲座的活性重组分组分为三种类型（I-IIII）。此外，我们与三种可食用的海藻物种新近隔离和描述了属于I型的讲座。我们发现，I-III型讲座具有抗HIV活性和抑制活性，以抵抗培养细胞的生长，这些细胞源自人类结肠癌。这些讲座对于轻松地将高甘露糖N-Glyans与彼此的混合物分开也很有用。

项目成果

藍藻Oscillatoriaagardhii由来抗HIVレクチンの遺伝子クローニング

蓝绿藻 Oscillatoriaagardhii 抗 HIV 凝集素的基因克隆

• 发表时间: 2008
• 作者: 今橋朋樹;狩野秀之;前田幸嗣;佐藤智美・佐々木ふみ・堀貫治
• 通讯作者: 佐藤智美・佐々木ふみ・堀貫治

Cloning, expression, and characterization of a novel anti-HIVlectin from the cultured cyanobacterium, Oscillatoriaagardhii.

来自培养蓝藻 Oscillatoriaagardhii 的新型抗 HIV 凝集素的克隆、表达和表征。

• 发表时间: 2009
• 期刊: Fish Sci 75
• 作者: Tomomi Sato;Kanji Hori
• 通讯作者: Kanji Hori

Isolation and characterization of a high mannose-type N-glycan specific lectin from the red alga Gracilariatikvahiae

红藻 Gracilariatikvahiae 中高甘露糖型 N-聚糖特异性凝集素的分离和表征

• 发表时间: 2008
• 作者: Hiromi Sibata;Kenji Egawa;Kanji Hori
• 通讯作者: Kanji Hori

Seasonal changes of growth rate and lectin contents in the red alga, Kappaphycusalvarezii cultivatedin Camranh Bay, Vietnam

越南金兰湾红藻生长速率和凝集素含量的季节变化

• 发表时间: 2008
• 作者: Hung Le Dinh;Kanji Hori;Nang Huynh Quang;Kha Tran
• 通讯作者: Kha Tran

Screening and preliminary characterizationof hemagglutinins in Vietnamese marine algae.

越南海藻中血凝素的筛选和初步表征。

• 发表时间: 2009
• 期刊: J ApplPhycol 21
• 作者: Hung Le Dinh;Kanj Hori;Nang Huynh Quang
• 通讯作者: Nang Huynh Quang