MECHANISM OF SUPPRESSION OF REPRODUCTIVE FUNCTION BY STRESS: ELECTROPHYSIOLOGICAL STUDY

压力抑制生殖功能的机制：电生理学研究

• 批准号: 03660308
• 负责人: NISHIHARA Masugi
• 金额: $ 1.34万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660308/
• 关键词: STRESS; GONADOTROPIN RELEASING HORMONE; PULSATILE SECRETION; HYPOTHALAMUS; PROSTAGLANDIN; LUTEINIZING HORMONE; TUMOR NECROSIS FACTOR; MULTIUNIT ACTIVITY; 多ニュ-ロン発射活動; 腫瘍壊死因子ーα

Under stress conditions, reproductive function is known to be suppressed. In the present study, the effect of tumor necrosis factor (TNF), which is secreted from macrophages and astrocytes in response to invasive stimuli, on the electrical activity of the hypothalamic gonadotropin-releasing hormone (GnRH) pulse generator was studied in the rat by means of multiunit activity (MUA) recording techniques. Characteristic increases (volleys) in MUA associated with pulsatile secretion of luteinizing hormone (LH). were recorded during a pretreatment control period. After either intravenous or intraventricular injection of TNF, MUA volleys occurred at regular intervals for about one hour, but thereafter the interval was prolonged for a few hours and then returned to control levels. These changes in MUA volleys were faithfully reflected by the pulsatile LH secretion. On the other hand, pretreatment with indomethacin blocked the effect of TNF on the interval between the MUA volleys. These results suggest that TNF, produced either in the peripheral tissue or in the brain, leads to a decrease in GnRH pulse frequency with a resultant suppression of reproductive function, and that the TNF action on hypothalamic GnRH pulse generator may be mediated by prostaglandins.

在压力条件下，生殖功能被抑制。本研究采用多单位电活动（MUA）记录技术，观察了巨噬细胞和星形胶质细胞对侵入性刺激产生的肿瘤坏死因子（TNF）对大鼠下丘脑促性腺激素释放激素（GnRH）脉冲发生器电活动的影响。与黄体生成素（LH）脉冲式分泌相关的MUA特征性增加（齐射）。在预处理对照期间记录。在静脉或脑室内注射TNF后，MUA以规则的间隔发生约1小时，但此后间隔延长数小时，然后恢复到对照水平。这些变化在MUA齐射忠实地反映了脉冲式LH分泌。另一方面，吲哚美辛预处理阻断了TNF对MUA放电间隔的影响。这些结果表明，TNF，无论是在外周组织或在大脑中产生，导致GnRH脉冲频率的降低，从而抑制生殖功能，和TNF的作用下丘脑GnRH脉冲发生器可能是由三尖杉酯碱介导的。

项目成果

KIMURA,F.,NISHIHARA,M.,HIRUMA.H.,FUNADASHI.T.: "Naioxone Increases the frequency of the electrical activity of Iuteinizing hoemone-releasing hormone pulse generator in long-term ovariectomized rats." Neuroendocrinoligy. 53. 97-102 (1991)

KIMURA,F.,NISHIHARA,M.,HIRUMA.H.,FUNADASHI.T.：“Naioxone 增加了长期卵巢切除大鼠中促黄体激素释放激素脉冲发生器的电活动频率。”

Nishihara,M.,Mori,Y.,Yoo,M.-J.,Takahashi,M.: "Pulsatility in Neuroendocrine Systems" Academic Press, (1993)

Nishihara,M.、Mori,Y.、Yoo,M.-J.、Takahashi,M.：“神经内分泌系统中的脉动”学术出版社，（1993）

T.Osada: "Sex differences in the basal firing rate and the responsiveness to opioid peptides of rat hippocampal neurons" Japanese Journal of Physiology. 41. 843-850 (1991)

T.Osada：“大鼠海马神经元基础放电率和阿片肽反应性的性别差异”日本生理学杂志。

Hiruma H, Nishihara M and Kimura F: "Hypothalamic electrical activity that relates to the pulsatile release of luteinizing hormone exhibits diurnal variation in ovariectomized rats." Brain Research. 582. 119-122 (1992)

Hiruma H、Nishihara M 和 Kimura F：“与黄体生成素脉冲式释放相关的下丘脑电活动在卵巢切除大鼠中表现出昼夜变化。”

西原 真杉、比留間 弘美、森 裕司: "視床下部電気活動のモニター法-GnRH分泌にともなう神経活動の変化-" 日本内分泌学会雑誌. 68. 1-10 (1992)

Masugi Nishihara、Hiromi Hiruma、Yuji Mori：“下丘脑电活动的监测方法 - 与 GnRH 分泌相关的神经活动的变化”日本内分泌学会杂志 68. 1-10 (1992)。