Study on the modulation of temperature signals from the skin by the nucleus raphe magnus and subcoeruleus

中缝大核和蓝下肌对皮肤温度信号调节的研究

基本信息

批准号：
03670082
负责人：
SATO Haruhiko
金额：
$ 1.28万
依托单位：
Chukyo Women's University (1992)Nagoya City University (1991)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

1. Extracellular recordings were made from dorsal horn cells responsive to temperature changes in the innocuous range (10-40ﾟC) of scrotal skin temperature in the lumbosacral segment of urethane-anesthetized rats. The temperature responsive dorsal horn cells (65) were classified into two groups : warm-responsive cells (48) showing an increase in firing rate by skin warming, warm-response, and cold-responsive cells (17) showing an increase in firing rate by skin cooing, cold-response. The effects of electrical stimulation in the nucleus raphe magnus (NRM) and subcoeruleus (SC) on their firing activity were studied.2. Effect of electrical stimulation in the NRM as well as in the SC was found to be characterized by a partial or complete depression of warm- or cold-responses. The background activity mostly remained unaffected.3. Depression of temperature response occurred in most (95%) of warm-responsive cells by NRM stimulation, whereas in the majority (79%) of cold-responsive cells by SC stimulation.4. In four warm-responsive cells, NRM-evoked depression of their temperature responses was partially antagonized by Serotonin antagonist, Methysergide (1 mg/kg, i.v.). Such NRM-evoked depression was suggested to be mediated by serotonergic raphe-spinal pathways.5. The results suggest that the raphe- and subcoeruleo-spinal pathways descendingly modulate the spinal transmission of warm and cold signals contributing to central thermoregulation.

1。细胞外记录是由背面喇叭细胞对尿氨酸 - 动力学大鼠的腰椎段中阴囊皮肤温度无害温度变化的反应。温度敏感的背角细胞（65）分为两组：温暖反应性细胞（48）显示皮肤变暖，温暖反应和冷响应性细胞的发射率提高（17），表明皮肤凝胶，冷响应，冷s的发射速率增加。研究了电刺激在raphe magnus（NRM）和亚焦点（SC）对发射活性中的影响。2。发现NRM以及SC中电刺激的影响的特征是部分或完全抑郁症的温暖或冷回答。背景活动主要不受影响。3。大多数（95％）通过NRM刺激发生了温度反应的抑郁症（95％），而在大多数（79％）的冷响应细胞中，SC刺激4。在四个温暖反应性的细胞中，NRM诱发的温度反应抑郁症由5-羟色胺拮抗剂甲基抗苷酸（1 mg/kg，i.v.）部分拮抗。这种NRM诱发的抑郁症被认为是由血清素能Raphe脊柱途径介导的。5。结果表明，raphe和coeruleo-Spinal途径下降会调节导致中央温度调节的温暖和冷信号的脊柱传播。