Study on the modulation of temperature signals from the skin by the nucleus raphe magnus and subcoeruleus

中缝大核和蓝下肌对皮肤温度信号调节的研究

• 批准号: 03670082
• 负责人: SATO Haruhiko
• 金额: $ 1.28万
• 依托单位: Chukyo Women's University (1992)Nagoya City University (1991)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670082/
• 关键词: dorsal horn neurons; temperature signals; raphe nucleus; subcoeruleus; serotonergic; noradrenergic; Methysergide; thermoregulation; 後角ニュ-ロン; 温度情報処理; 脊髄伝達; セロトニン作動性神経路; ノルアドレナリン作動性神経路

1. Extracellular recordings were made from dorsal horn cells responsive to temperature changes in the innocuous range (10-40ﾟC) of scrotal skin temperature in the lumbosacral segment of urethane-anesthetized rats. The temperature responsive dorsal horn cells (65) were classified into two groups : warm-responsive cells (48) showing an increase in firing rate by skin warming, warm-response, and cold-responsive cells (17) showing an increase in firing rate by skin cooing, cold-response. The effects of electrical stimulation in the nucleus raphe magnus (NRM) and subcoeruleus (SC) on their firing activity were studied.2. Effect of electrical stimulation in the NRM as well as in the SC was found to be characterized by a partial or complete depression of warm- or cold-responses. The background activity mostly remained unaffected.3. Depression of temperature response occurred in most (95%) of warm-responsive cells by NRM stimulation, whereas in the majority (79%) of cold-responsive cells by SC stimulation.4. In four warm-responsive cells, NRM-evoked depression of their temperature responses was partially antagonized by Serotonin antagonist, Methysergide (1 mg/kg, i.v.). Such NRM-evoked depression was suggested to be mediated by serotonergic raphe-spinal pathways.5. The results suggest that the raphe- and subcoeruleo-spinal pathways descendingly modulate the spinal transmission of warm and cold signals contributing to central thermoregulation.

1.在麻醉大鼠的腰骶段，背角细胞对阴囊皮肤温度的无害范围（10-40 ℃）内的温度变化做出反应，进行细胞外记录。将温度响应背角细胞（65）分为两组：显示出通过皮肤加温而放电率增加的温响应细胞（48），温响应，和显示出通过皮肤冷却而放电率增加的冷响应细胞（17），冷响应。研究了电刺激中缝大核（NRM）和蓝斑下核（SC）对其放电活动的影响.研究发现，电刺激对NRM和SC的影响的特征是部分或完全抑制热反应或冷反应。背景活动基本保持不受影响。大部分（95%）的温反应细胞受到NRM刺激后温度反应降低，而大部分（79%）的冷反应细胞受到SC刺激后温度反应降低.在四个温反应细胞中，NRM引起的温度反应的抑制被5-羟色胺拮抗剂Methysergide（1 mg/kg，i. v.）部分拮抗。这种NRM诱发的抑制被认为是由多巴胺能中缝-脊髓通路介导的。结果表明，中缝和subcoeruleo脊髓通路下行调制脊髓传递的温暖和寒冷的信号，有助于中央温度调节。

项目成果

Haruhiko SATO and Hitoo NISHINO: "Raphe-spinal and subcoeruleo-spinal modulat-ion of temperature signal tranasmission in rats." Abstracts of 20th Annual Meeting of Ameri-can Society for Neuroscience at St.Louis. P.96 (1990)

Haruhiko SATO 和 Hitoo NISHINO：“大鼠温度信号传递的中缝脊髓和蓝核下脊髓调节。”

Haruhiko Sato et al.: "Temperature signal modulation by raphe- and subcoeruleo-spinal pathways of rats." Jap. J. Physiol.41. S330 (1991)

Haruhiko Sato 等人：“大鼠中缝和蓝核下脊髓通路的温度信号调节。”

Sato,H. et al.: "Modulation of temperature signal transmission by raphe-spinal and subcoeruleo-spinal pathways in rats" Neurosci. Res. Suppl. 14. S160 (1991)

佐藤，H.

Haruhiko Sato: "Modulation of thermoafferent signals and thermoregulatory response by brain stem." in Symposium on Thermoregulation and related systems in 70th Congress of the Physiological Society of Japan,Yamanashi. April. (1993)

Haruhiko Sato：“脑干对热传入信号和温度调节反应的调节。”

Haruhiko Sato et al.: "Descending modulation of temperature signals by the nucleus raphe magnus and subcoeruleus in rats." Jap. J. Physiol.40. S281 (1990)

Haruhiko Sato 等人：“大鼠中缝大核和蓝下核对温度信号的降序调节。”