New vasoactive peptides and their functions

(1) 开发新的血管活性肽的调查方法：除了我们以前对几种平滑肌制剂使用收缩或松弛活性的方法之外，我们开发了一种新方法，测量培养的血管平滑肌细胞和内皮细胞中的细胞中的第二信使，例如 cAMP 和 cGMP。 (2) THP-1 细胞系中 CNP 的产生：我们在添加了佛波醇酯 (TPA) 的培养基中检测到了强烈的小鸡直肠松弛活性单核细胞白血病细胞系，THP-1。我们从培养基中纯化出具有直肠松弛活性的肽。经结构分析确定该肽为CNP-29。由于血管平滑肌细胞(VSMC)表达CNP特异性GC-B受体，单核细胞(或巨噬细胞)来源的CNP可能调节VSMC功能。(3)C型利钠肽(CNP)：CNP已被认为是在中枢神经系统中发挥作用的神经肽。通过PCR方法和高灵敏度的RIA方法，我们在外周组织中发现了CNP及其mRNA，包括主动脉、肾脏、肺、胃肠道。这些结果表明 CNP 可能是通过旁分泌模式发挥作用的局部调节剂。

(1) Development of survey methods for new vasoactive peptides : In addition to our previous methods using contractile or relaxant activity on several smooth muscle preparations, We developed a new method measuring second messengers in the cell, such as cAMP and cGMP, in cultured vascular smooth muscle cells and endothelial cells.(2) production of CNP in THP-1 cell line : We detected strong chick rectum relaxant activity in phorbol-ester (TPA) supplemented culture medium of monocytic leukemia cell line, THP-1. From the culture medium, we purified a peptide exhibiting rectum relaxant activity. The peptide was determined to be CNP-29 by structural analyses.Since vascular smooth muscle cells (VSMC) express CNP-specific GC-B receptor, CNP derived from monocytes (or macrophages) may regulate VSMC functions.(3) C-type natriuretic peptide (CNP) : CNP has been recognized as a neuropeptide functioning in the central nervous system. By using the PCR method and highly sensitive RIA method, we found CNP and its mRNA in the peripheral tissue, including aorta, kidney, lung, gastrointestinal tract. These results suggest that CNP may be a local regulator functioning through paracrine mode.