Tissue-specific processing pathway of precursor proteins deduced from peptidome analysis

点击翻译按钮获取中文摘要

Some proteins are post-translationally converted to peptides,which in turn function as hormones and neuropeptides. This conversion reaction is called "processing", and tissue-specifically performed by a set of processing enzymes. Peptides generated from the same precursor protein are often different in each tissue, and hard to predict from genome information. In this study, we prepared intact peptides from endocrine-type tissues and cells, and comprehensively identified them by a renovated peptidomic technology. Based on accumulated peptide data of these cells and tissues, we provide a principal approach to deduce a processing pathway of a particular precursor protein as well as peptides generated from the protein in target cells or tissues.