Tissue-specific processing pathway of precursor proteins deduced from peptidome analysis

从肽组分析推导出前体蛋白的组织特异性加工途径

• 批准号: 18310138
• 负责人: MINAMINO Naoto
• 金额: $ 11.78万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18310138/
• 关键词: ペプチドーム; プロセシング; 質量分析; 下垂体; 甲状腺C細胞; ペプチド; 生理活性ペプチド; カルシトニン; カルシトニン遺伝子関連ペプチド; 分解ペプチド; 副腎髄質; データベース

Some proteins are post-translationally converted to peptides,which in turn function as hormones and neuropeptides. This conversion reaction is called "processing", and tissue-specifically performed by a set of processing enzymes. Peptides generated from the same precursor protein are often different in each tissue, and hard to predict from genome information. In this study, we prepared intact peptides from endocrine-type tissues and cells, and comprehensively identified them by a renovated peptidomic technology. Based on accumulated peptide data of these cells and tissues, we provide a principal approach to deduce a processing pathway of a particular precursor protein as well as peptides generated from the protein in target cells or tissues.

一些蛋白质在代谢后转化为肽，肽又作为激素和神经肽发挥作用。这种转化反应被称为“加工”，并且由一组加工酶组织特异性地进行。由相同的前体蛋白产生的肽在每个组织中通常是不同的，并且很难从基因组信息中预测。在本研究中，我们从内分泌型组织和细胞中制备了完整的肽，并通过更新的肽组学技术对其进行了全面的鉴定。基于这些细胞和组织积累的肽数据，我们提供了一种主要的方法来推断特定前体蛋白的加工途径以及由靶细胞或组织中的蛋白质产生的肽。

项目成果

C-type natriuretic peptide is specifically augmented by pituitary adenylate cyclase-activating polypeptide in rat astrocytes.

大鼠星形胶质细胞中的垂体腺苷酸环化酶激活多肽可特异性增强 C 型利钠肽。

• 发表时间: 2006
• 期刊: Ann. N. Y. Acad. Sci. 1070
• 作者: Fujikawa K;Minamino N;Miyata A;et. al.
• 通讯作者: et. al.

Snapshot Peptidomics of the Regulated Secretory Pathway*

• DOI: 10.1074/mcp.m900044-mcp200
• 发表时间: 2009-03
• 期刊: Molecular & Cellular Proteomics
• 影响因子: 7
• 作者: K. Sasaki;Y. Satomi;T. Takao;N. Minamino

A novel member of the calcitonin gene-related peptide family, calcitonin receptor-stimulating peptide, inhibits the formation and activity of osteoclasts

• DOI: 10.1016/j.ejphar.2007.01.034
• 发表时间: 2007-04-10
• 期刊: EUROPEAN JOURNAL OF PHARMACOLOGY
• 影响因子: 5
• 作者: Notoya, Michitaka;Arai, Rumiko;Hagiwara, Hiromi
• 通讯作者: Hagiwara, Hiromi

Distribution of neuroendocrine regulatory peptide-1 and -2, and proteolytic processing of their precursor VGF protein in the rat.

神经内分泌调节肽-1和-2的分布及其前体VGF蛋白在大鼠中的蛋白水解加工。

• 发表时间: 2010
• 期刊: J. Neurochem 114
• 作者: E. Mishiro-Satoh;K. Sasaki;T. Matsuo;H. Kageyama;H. Yamaguchi;Y. Date;M. Matsubara;T. Ishizu;K. Yoshizawa-Kumagaye;Y. Satomi;T. Takao;S. Shioda;M. Nakazato;N. Minamino
• 通讯作者: N. Minamino

Identification and biological activity of ovine and caprine calcitonin receptor-stimulatine peptides 1 and 2

绵羊和山羊降钙素受体刺激肽 1 和 2 的鉴定及其生物活性

• 发表时间: 2008
• 期刊: J. Endocrinol 198
• 作者: Charles CJ;Katafuchi T;Yandle TG;Minamino N.
• 通讯作者: Minamino N.