Roles of complement and complement receptor in self defense

补体和补体受体在自卫中的作用

• 批准号: 03670212
• 负责人: KINOSHITA Taroh
• 金额: $ 1.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670212/
• 关键词: Self defense; Complement; Complement receptor; 補体レセプタ-

Complement is activated on the surface of microorganisms antibody-dependently and -independently. C3b molecules are deposited on the microbial surfaces as clusters. The C3b molecules are subsequently degraded to iC3b and then to C3dg. These C3 fragments act as ligands of complement receptors expressed on various cells that participate in self defense system. This study aimed to elncidate roles of complement and complement receptors in the self defense system. We made following findings.1. Within the classical pathway C5 convertase C3b binds to specific Ser residue of C4b (Ser 1217 in the human and Ser 1213 in the mouse).2. We evaluated roles of complement receptors CR1 and CR2 in the humoral immune response by injecting mice with monoclonal antibody (7G6) that is capable of down-modulating mouse CR1 and CR2 on B-lymphocytes and of inhibiting C3d-binding to these receptors. Animals treated with 7G6 responded very weakly to the limited doses of thymus-dependent and -independent antigens. These results elarified important roles of complement receptors in antibody response.

补体在微生物表面的激活依赖于抗体，也不依赖于抗体。C3b分子以簇状沉积在微生物表面。C3b分子随后被降解成ICC3b，然后再降解成C3dg。这些C3片段作为补体受体的配体，表达在参与自我防御系统的各种细胞上。本研究旨在探讨补体和补体受体在自我防御系统中的作用。我们取得了以下发现：1.在经典途径C5中，C3b结合到C4b的特定Ser残基(人中的Ser1217和小鼠中的Ser1213)。我们通过给小鼠注射能够下调小鼠B淋巴细胞上CR1和CR2的单抗(7G6)并抑制C3d与这些受体的结合来评价补体受体CR1和CR2在体液免疫反应中的作用。用7G6处理的动物对有限剂量的胸腺依赖和非依赖抗原的反应非常弱。这些结果阐明了补体受体在抗体反应中的重要作用。

项目成果

Thyphronitis, E: "Modulation of mouse complement receptor 1 and 2 in vivo suppress antibody responses" J. Immunol. 147. 224-230 (1991)

甲状腺炎，E：“体内调节小鼠补体受体 1 和 2 抑制抗体反应”J.Immunol。

G.Thyphronitis: "Modulation of mouse complement receptors 1 and 2 suppresses antibody responses in Vino." J.Immunol.147. 224-230 (1991)

G. 甲状腺炎：“调节小鼠补体受体 1 和 2 可抑制 Vino 中的抗体反应。”

Youn Uck Kim: "Covalent finding of C3b to C4b within the classical complement pathway C5 corvertase." J.Biol.Chem.267. (1992)

Youn Uck Kim：“经典补体途径 C5 皮质酶内 C3b 与 C4b 的共价发现。”

KINOSHITA,T.: "Biology of complement:the overture." Immunol.Today. 12. 291-295 (1991)

KINOSHITA,T.：“补体生物学：序曲。”

MORINA,H.: "Distinct rceptor and regulatory properties of recombinant mouse complement receptor 1(CR1)and Crry,the two genetic homologs of human CR1." J.Exp.Med.175. 121-129 (1992)

MORINA, H.：“重组小鼠补体受体 1 (CR1) 和 Crry（人类 CR1 的两种基因同源物）的独特受体和调节特性。”