Molecular mechanisms and functions of structural changes of protein GPI-anchors

蛋白质GPI锚结构变化的分子机制和功能

• 批准号: 21247018
• 负责人: KINOSHITA Taroh
• 金额: $ 28.79万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21247018/
• 关键词: GPIアンカー; 遺伝子; 酵素; タンパク質; 糖鎖; 糖質

GPI-anchor is a glycolipid acts as a membrane anchor of many eukaryotic cell surface proteins. In this study, we aimed to clarify (1) mechanisms and biological significance of fatty acid remodeling of GPI-anchored proteins (GPI-AP); (2) mechanism and biological significance of 1-alkyl, 2-acyl form of GPI anchors; and (3) roles of GPI structural remodeling in transport of GPI-AP. We found (1) that GPI fatty acid remodeling is important for homeostasis of humoral immunity; (2) that 1-alkyl, 2-acyl form of GPI anchors is defective in major peroxisome disorders; and (3) that proper remodeling of GPI-AP is critical for association with their cargo receptors and efficient transport.

GPI-锚是一种糖脂，作为许多真核细胞表面蛋白的膜锚。本研究旨在阐明GPI锚定蛋白（GPI-AP）脂肪酸重构的机制和生物学意义; GPI锚定蛋白1-烷基、2-酰基形式的机制和生物学意义; GPI结构重构在GPI-AP转运中的作用。我们发现（1）GPI脂肪酸重塑对于体液免疫的稳态很重要;（2）GPI锚的1-烷基，2-酰基形式在主要过氧化物酶体疾病中是有缺陷的;（3）GPI-AP的适当重塑对于与其货物受体和有效运输的关联至关重要。

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GPIアンカー型タンパク質の切断に関与する新規酵素の機能解析

参与 GPI 锚定蛋白裂解的新型酶的功能分析

• 发表时间: 2012
• 作者: Kinoshita;T.;Y. Maeda and M. Fujita;Morihisa Fujita;Morihisa Fujita;Morihisa Fujita;藤田盛久;Morihisa Fujita;藤田盛久
• 通讯作者: 藤田盛久

GPI Glycan Remodeling by PGAP5 Regulates Transport of GPI-Anchored Proteins from the ER to the Golgi

• DOI: 10.1016/j.cell.2009.08.040
• 发表时间: 2009-10-16
• 期刊: CELL
• 影响因子: 64.5
• 作者: Fujita, Morihisa;Maeda, Yusuke;Kinoshita, Taroh
• 通讯作者: Kinoshita, Taroh

Deficiencies of GPI mannosyltransferases 1 and 2 cause different fates of GPI anchored proteins

GPI 甘露糖基转移酶 1 和 2 的缺陷导致 GPI 锚定蛋白的不同命运

• 发表时间: 2012
• 作者: Fukagawa A;Hiroshima M;Tokunaga M;Taroh Kinoshita
• 通讯作者: Taroh Kinoshita

Remodeling of GPI-ancherd protein during biosynthesis in transport for the ER.

内质网运输生物合成过程中 GPI 锚定蛋白的重塑。

• 发表时间: 2010
• 作者: Hiraoka;D.;Okumura;E. and Kishimoto;T.;岩崎博史;Taroh Kinoshita
• 通讯作者: Taroh Kinoshita