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Studies on agglutinating antibodies of group O serum against weak subgroup red cells

O型血清抗弱亚群红细胞凝集抗体的研究

基本信息

批准号：
03670302
负责人：
AGO Kazutoshi
金额：
$ 1.09万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670302/
关键词：
Forensic serology ABO blood group system Group O serum Cross-reacting antibody Weak subgroup Affinity chromatography IgG antibody 交叉反応性抗体アフイニテイクロマトグラフイー抗A,B抗体変異型アフイニテイ精製

项目摘要

The basic cause of superior agglutinating activities of group O serum against weak subgroup red cells was investigated. Blood group specific antibodies in group O, group A, and group B serum were fractionated by affinity chromatography with group A and group B specific immunoadsorbents. The antibody fractions obtained were tested for their strength against A_3B, Ax, Bm, and ABm cells, respectively. All of the anti-A, anti-B, and anti-A, B from group O serum were found to be highly effective agglutinators of weak subgroup cells in contrast to both of anti-A from group B and anti-B from group A serum. These findings suggest that anti-A, anti-B, and anti-A, B antibodies in group O serum take part in serological reactions leading to the agglutination of weak subgroup cells together. Furthermore, studies by the immunodiffusion method and the mercaptoethanol method revealed that all of the antibodies from group O serum were IgG antibodies, whereas those from group A and group B serum were IgM antibodies. Thus, it can be concluded that the superior agglutinating activities of group O serum against weak subgroup cells are attributed to IgG anti-A, IgG anti-B, and IgG anti-A, B antibodies present in the serum. Although the absorption test has been conducted with several kinds of synthetic origosaccharides (Synsorb) relevant to the ABO blood group system, whether the structures of group specific antigens recognized by the antibodies from group O serum differ from those by antibodies from group A and group B serum can not be decided at the present time.

本文探讨了O型血清对弱亚群红细胞具有上级凝集活性的根本原因。用A、B型特异性免疫吸附剂亲和层析分离O、A、B型血清中的血型特异性抗体。分别检测了所获得的抗体组分对A_3B、Ax、Bm和ABm细胞的抗肿瘤强度。O群抗A、抗B和抗A、抗B血清均能高效凝集弱亚群细胞，而A群抗B和抗B血清均不能凝集弱亚群细胞。提示O型血清中抗A、抗B和抗A、抗B抗体参与了弱亚群细胞凝集的血清学反应。此外，免疫扩散法和巯基乙醇法的研究表明，O组血清中的抗体均为IgG抗体，而A组和B组血清中的抗体均为IgM抗体。因此，可以得出结论，O型血清对弱亚群细胞的上级凝集活性归因于血清中存在的IgG抗A、IgG抗B和IgG抗A、B抗体。尽管已经用与ABO血型系统相关的几种合成的原卟啉（SynsorB）进行了吸收试验，但目前还不能确定O型血清抗体识别的群特异性抗原的结构是否不同于A型和B型血清抗体识别的群特异性抗原的结构。