Mechanism of primary biliary cirrhosis and free radicals

原发性胆汁性肝硬化与自由基的机制

• 批准号: 03670342
• 负责人: SEKIYA Chihiro
• 金额: $ 1.34万
• 依托单位: Asahikawa Medica College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670342/
• 关键词: primary biliary cirrhosis; free radicals; manganese-superoxide dismutase; Mn‐SOD; Mnーsuperoxide dismutase; 活性酸素; フリ-ラジカル; サイトカイン

Serum manganese-superoxide dismutase (Mn-SOD) was determined in patients with various liver diseases with an enzyme-linked immunosorbent assay using a specific monoclonal antibody.The serum Mn-SOD level in patients with primary biliary cirrhosis (PBC) was significantly increased compared with those of other liver diseases, including chronic viral hepatitis, liver cirrhosis, autoimmune hepatitis, obstructive jaundice, fatty liver and idiopathic portal hypertension. Administration of ursodeoxycholic acid caused the significant decrease of serum Mn-SOD level in PBC patients. Immunostaining of a liver biopsy specimen from the patients with PBC revealed increased expression of the enzyme protein in damaged hepatocytes and degenerated epithelial cells of interlobular bile ducts.

在患有各种肝疾病患者的患者中，使用特定的单克隆抗体的血清型锰 - 氧化酶蒸馏酶（MN-SOD）确定。在原发性胆汁性cirrhosis（pbc）中，血清MN-SOD水平显着增加，与其他Liver Disiver相比，肝脏中胆汁虫（PBC）显着升高。自身免疫性肝炎，阻塞性黄疸，脂肪肝和特发性门户高血压。 Ursodoxycholic的给药导致PBC患者血清MN-SOD水平的显着降低。对PBC患者的肝活检标本的免疫染色显示，酶蛋白在受损的肝细胞受损的酶蛋白表达增加和囊肿间胆管的退化上皮细胞。

项目成果

Ono M: "Elevated level of serum Mn-superoxide dismutasse in patients with primary biliary clrrhosis : possible involvement of free radicals in the pathogenesis in primary biliary cirrhosis." J Lab Clin Med. 118. 476-483 (1991)

Ono M：“原发性胆汁性肝硬化患者血清锰超氧化物歧化酶水平升高：自由基可能参与原发性胆汁性肝硬化的发病机制。”

OHHIRA M.: "Primary biliary cirrhosis and manganese-superoxide dismutase." Jpn J Gastroenterol. 89. 1474 (1992)

OHHIRA M.：“原发性胆汁性肝硬化和锰超氧化物歧化酶。”

ONO M.: "Elevated level of serum Mn-supweoxide dismutase in patients with primary biliary cirrhosis : Possible involvement of free radicals in the pathogenesis in primary biliary cirrhosis." J Lab Clin Med. 118. 476-483 (1991)

ONO M.：“原发性胆汁性肝硬化患者血清锰超氧化物歧化酶水平升高：自由基可能参与原发性胆汁性肝硬化的发病机制。”

Ono M,et al.: "Elevated level of serum Mnーsuperoxide dismutase in patients with primary biliary cirrhosisーpossible involvement of free radicals in the pathogenesis of primary biliary cirrhosis" J Lab Clin Med. 118. 476-483 (1991)

Ono M 等人：“原发性胆汁性肝硬化患者血清锰超氧化物歧化酶水平升高——自由基可能参与原发性胆汁性肝硬化的发病机制”J Lab Clin Med 118. 476-483 (1991)。

大平 基之: "PBC患者における血清Mn-SOD:UDCA投与による変動" 肝臓. 33. 811- (1992)

Motoyuki Ohira：“PBC 患者血清 Mn-SOD：UDCA 给药引起的变化”肝脏。33. 811- (1992)