Interactions of intracellular messengers of amylase secretion in pancreatic acini

胰腺腺泡中淀粉酶分泌的细胞内信使的相互作用

• 批准号: 03670356
• 负责人: HAYAKAWA Tetsuo
• 金额: $ 1.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670356/
• 关键词: exocrine secretion; pancreatic acini; intracellular messengers; permeabilized cell; calmodulin; calmodulin kinase; W7; KN62; 酵素分泌; 細胞内メッセンジャ-; Cキナ-ゼ; G蛋白質

To investigate the interactions of intracellular messengers of amylase secretion in stimulus-secretion coupling in pancreatic acini, we studied the effects of A23187, TPA,dbcAMP on amylase secretion. These experiments reveal that Ca^<2+> plays a crucial role in the interactions of intracellular mediators. Then we studied the effect of W7, a calmodulin inhibitor, and KN62, a specific inhibitor of calmodulindependent protein kinase II, on amylase secretion to investigate the role of calmodulin and calmodulin-dependent kinase in Ca^<2+>-dependent amylase secretion. W7 and KN62 reduced amylase secretion stimulated by CCK. W7 and KN62 did not inhibit amylase secretion stimulated by A23187 or TPA, but reduced a synergistic secretion by both A23187 and TPA. W7 and KM62 had no effects on Ca^<2+>-dependent amylase secretion from permeabilized acini, but inhibited the enhancement of Ca^<2+>-dependent amylase secretion by GTPyS, TPA or cAMP. These data suggest that calmodulin plays an important role in Ca^<2+>-dependent amylase secretion from pancreatic acinar cells and in the interactions between Ca^<2+> and other intracellular messengers.

为探讨胰腺腺泡细胞内淀粉酶分泌信使在刺激-分泌偶联中的相互作用，我们研究了A23187、TPA、DBcAMP对胰腺腺泡细胞淀粉酶分泌的影响。这些实验表明，钙离子在细胞内介质的相互作用中起着至关重要的作用。然后，我们研究了钙调蛋白抑制剂W7和钙调素非依赖性蛋白激酶II的特异性抑制剂KN62对淀粉酶分泌的影响，以探讨钙调蛋白和钙调蛋白依赖的激酶在钙依赖的淀粉酶分泌中的作用。W7和KN62抑制CCK刺激的淀粉酶分泌。W7和KN62不抑制A23187或TPA刺激的淀粉酶分泌，但抑制A23187和TPA的协同分泌。W7和KM62对通透性腺泡分泌依赖于Ca~(2+)的淀粉酶无影响，但可抑制GTPyS、TPA或cAMP对依赖于Ca~(2+)的淀粉酶分泌的促进作用。这些结果表明，钙调蛋白在胰腺腺泡细胞钙依赖的淀粉酶的分泌以及钙离子与其他细胞内信使的相互作用中起重要作用。

项目成果

Ishiguro H,Hayakawa T,et al.: "The effects of calmodulin inhibitor on amylase secretion from rat pancreatic acini" Am J Physiology.

Ishiguro H、Hayakawa T 等人：“钙调蛋白抑制剂对大鼠胰腺腺泡淀粉酶分泌的影响”Am J Physiology。

北川 元二 早川 哲夫、他: "膵腺房細胞における細胞内メッセンジャーの相互作用" 病態病理. 12. (1993)

Genji Kitakawa、Tetsuo Hayakawa 等人：“胰腺腺泡细胞中细胞内信使的相互作用”病理病理学 12。

Ishiguro H,Hayakawa T, et al: "Effects of calmodulin inhibitors on amylase secretion from rat pancreatic acini" Digestion. 53. (1993)

Ishiguro H、Hayakawa T 等人：“钙调蛋白抑制剂对大鼠胰腺腺泡淀粉酶分泌的影响”消化。

Ishiguro H,Hayakawa T,et al: "Effects of calmodulin inhibitors on amylase secretion from rat pancreatic acini." Digestion. 53. (1993)

Ishiguro H、Hayakawa T 等人：“钙调蛋白抑制剂对大鼠胰腺腺泡淀粉酶分泌的影响”。

Ishiguro H,Hayakawa T,etal: "Effects of calmodulin inhibitors on amylase secretion from rat pancreatic acini" Digestion. 53. (1993)

Ishiguro H，Hayakawa T，etal：“钙调蛋白抑制剂对大鼠胰腺腺泡淀粉酶分泌的影响”消化。